SARS-CoV-2 Proteome Microarray for Mapping COVID-19 Antibody Interactions at Amino Acid Resolution
Top Cited Papers
- 21 October 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in ACS Central Science
- Vol. 6 (12), 2238-2249
- https://doi.org/10.1021/acscentsci.0c00742
Abstract
Comprehensive profiling of humoral antibody response to severe acute respiratory syndrome (SARS) coronavirus-2 (CoV-2) proteins is essential in understanding the host immunity and in developing diagnostic tests and vaccines. To address this concern, we developed a SARS-CoV-2 proteome peptide microarray to analyze antibody interactions at the amino acid resolution. With the array, we demonstrate the feasibility of employing SARS-CoV-1 antibodies to detect the SARS-CoV-2 nucleocapsid phosphoprotein. The first landscape of B-cell epitopes for SARS-CoV-2 IgM and IgG antibodies in the serum of 10 coronavirus disease of 2019 (COVID-19) patients with early infection is also constructed. With array data and structural analysis, a peptide epitope for neutralizing antibodies within the SARS-CoV-2 spike receptor-binding domain’s interaction interface with the angiotensin-converting enzyme 2 receptor was predicted. All the results demonstrate the utility of our microarray as a platform to determine the changes of antibody responses in COVID-19 patients and animal models as well as to identify potential targets for diagnosis and treatment.Funding Information
- Ministry of Science and Technology of the People's Republic of China (2017YFC0906703, 2018YFA0507503, 2018ZX09733003, 2020YFE0202200)
- Beijing Municipal Commission of Education
- Chinese Academy of Medical Sciences (2017-I2M-3-001, 2017-I2M-B&R-01)
- National Natural Science Foundation of China (31870823, 81671618, 81673040, 81871302)
- State Key Laboratory of Proteomics, Academy of Military Medical Sciences (SKLP-C202001, SKLP-K201505, SKLP-O201703)
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