Effects of Pathological Flow on Pulmonary Artery Endothelial Production of Vasoactive Mediators and Growth Factors
- 30 June 2009
- journal article
- research article
- Published by S. Karger AG in Journal of Vascular Research
- Vol. 46 (6), 561-571
- https://doi.org/10.1159/000226224
Abstract
Background: Alterations in pulmonary blood flow are often associated with the initiation and progression of pulmonary vascular disease. However, the cellular mechanisms involved in mediating flow effects in the pulmonary circulation remain unclear. Depending on the disease condition, flow may be extremely low or high. We therefore examined effects of pathologically low and high flow on endothelial production of factors capable of affecting pulmonary vascular tone and structure as well as on potential underlying mechanisms. Methods: Flow effects on pulmonary endothelial release of NO, PGF1a, ET-1 and TxB2, on expression of total and phosphorylated eNOS as well as Akt, and on VEGF were examined. Additionally, in a coculture system, effects of flow-exposed endothelial cells on smooth muscle (SM) proliferation and contractile protein were studied. Results: Compared to physiological flow, pathologically high and low flow attenuated endothelial release of NO and PGF1a, and enhanced release of ET-1. Physiological flow activated the Akt/eNOS pathway, while pathological flow depressed it. Pathologically high flow altered VE-cadherin expression. Pathologically high flow on the endothelium upregulated α-SM-actin and SM-MHC without affecting SM proliferation. Conclusion: Physiological flow leads to production of mediators which favor vasodilation. Pathological flow alters the balance of mediator production which favors vasoconstriction.Keywords
This publication has 30 references indexed in Scilit:
- Electrical stimulation directly induces pre-angiogenic responses in vascular endothelial cells by signaling through VEGF receptorsJournal of Cell Science, 2004
- Bovine distal pulmonary arterial media is composed of a uniform population of well-differentiated smooth muscle cells with low proliferative capabilitiesAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2003
- Flow-dependent regulation of endothelial nitric oxide synthase: role of protein kinasesAmerican Journal of Physiology-Cell Physiology, 2003
- Developmental differences in the shear stress-induced expression of endothelial NO synthase: changing role of AP-1American Journal of Physiology-Lung Cellular and Molecular Physiology, 2003
- Mature Vascular Endothelium Can Give Rise to Smooth Muscle Cells via Endothelial-Mesenchymal TransdifferentiationCirculation Research, 2002
- Hemodynamic Shear Stress and Its Role in AtherosclerosisJAMA, 1999
- Influence of Oscillatory and Unidirectional Flow Environments on the Expression of Endothelin and Nitric Oxide Synthase in Cultured Endothelial CellsArteriosclerosis, Thrombosis, and Vascular Biology, 1998
- Cerebral hemodynamics and distribution of left ventricular output during inhalation of nitric oxideCritical Care Medicine, 1995
- Effects of Pulsatile Flow on Cultured Vascular Endothelial Cell MorphologyJournal of Biomechanical Engineering, 1991
- Changes in pulmonary blood flow affect vascular response to chronic hypoxia in rats.Circulation Research, 1983