EVIDENCE OF INFLAMMATORY CELL INVOLVEMENT IN BRAIN ARTERIOVENOUS MALFORMATIONS
- 1 June 2008
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Neurosurgery
- Vol. 62 (6), 1340-1350
- https://doi.org/10.1227/01.neu.0000333306.64683.b5
Abstract
Brain arteriovenous malformations (AVM) have high matrix metalloproteinase-9, interleukin-6, and myeloperoxidase (MPO) expression, and polymorphic variations in inflammatory genes are associated with an increased risk of hemorrhage. In this study, we characterized the presence of inflammatory cells in AVM lesional tissue specimens. Immunohistochemistry was used to identify and localize neutrophils (MPO as marker), macrophages/microglia (CD68 as marker), T lymphocytes (CD3 as marker), and B lymphocytes (CD20 as marker). Endothelial cell (EC) marker CD31 was used as an index to assess vascular mass (EC mass). Surgical specimens from 20 unruptured, nonembolized AVMs were examined; seven cortical samples from temporal lobectomy were used as controls. Positive signals for inflammatory cell markers were counted and analyzed by normalizing to the area of the tissue section and the amount of endothelial cells (cells/mm2/EC mass pixels). Levels of MPO and matrix metalloproteinase 9 were determined by enzyme-linked immunosorbent assay. Neutrophils and macrophages are all frequently identified in the vascular wall of AVM tissue. In contrast, T and B lymphocytes are rarely observed in AVM tissue. AVM tissue displayed more neutrophil and macrophage/microglia markers than epilepsy control tissue (MPO: 434 ± 333 versus 5 ± 4, P = 0.0001; CD68: 454 ± 404 versus 4 ± 2, P = 0.0001; cells/mm2/EC mass pixels). In ex vivo studies, neutrophil quantity, MPO, and matrix metalloproteinase-9 levels were all colinear (R 2 = 0.98–0.99). Our study demonstrates that inflammatory cells are present in AVM tissue. Taken together with previous genetic and cytokine studies, these data are consistent with a novel view that inflammation is associated with AVM disease progression and rupture.Keywords
This publication has 47 references indexed in Scilit:
- Astrocytic Induction of Matrix Metalloproteinase-9 and Edema in Brain HemorrhageJournal of Cerebral Blood Flow & Metabolism, 2006
- Intracranial aneurysms: links among inflammation, hemodynamics and vascular remodelingNeurological Research, 2006
- Tumor Necrosis Factor-α–238G>A Promoter Polymorphism Is Associated With Increased Risk of New Hemorrhage in the Natural Course of Patients With Brain Arteriovenous MalformationsStroke, 2006
- Interleukin‐6 involvement in brain arteriovenous malformationsAnnals of Neurology, 2005
- Abnormal Expression of Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases in Brain Arteriovenous MalformationsStroke, 2003
- Characteristics of Brain Arteriovenous Malformations With Coexisting AneurysmsStroke, 2002
- Myeloperoxidase, a catalyst for lipoprotein oxidation, is expressed in human atherosclerotic lesions.JCI Insight, 1994
- Cd20 (pan‐B cell) antigen is expressed at a low level on a subpopulation of human T lymphocytesCytometry, 1993
- Polymorphonuclear leukocytes occlude capillaries following middle cerebral artery occlusion and reperfusion in baboons.Stroke, 1991
- Leukocytes and Ischemia-Induced Myocardial InjuryAnnual Review of Pharmacology and Toxicology, 1986