Biomarkers of Chlorpyrifos Exposure and Effect in Egyptian Cotton Field Workers
- 1 June 2011
- journal article
- Published by Environmental Health Perspectives in Environmental Health Perspectives
- Vol. 119 (6), 801-806
- https://doi.org/10.1289/ehp.1002873
Abstract
Chlorpyrifos (CPF), a widely used organophosphorus pesticide (OP), is metabolized to CPF-oxon, a potent cholinesterase (ChE) inhibitor, and trichloro-2-pyridinol (TCPy). Urinary TCPy is often used as a biomarker for CPF exposure, whereas blood ChE activity is considered an indicator of CPF toxicity. However, whether these biomarkers are dose related has not been studied extensively in populations with repeated daily OP exposures. We sought to determine the relationship between blood ChE and urinary TCPy during repeated occupational exposures to CPF. Daily urine samples and weekly blood samples were collected from pesticide workers (n = 38) in Menoufia Governorate, Egypt, before, during, and after 9–17 consecutive days of CPF application to cotton fields. We compared blood butyrylcholinesterase (BuChE) and acetylcholinesterase (AChE) activities with the respective urinary TCPy concentrations in each worker. Average TCPy levels during the middle of a 1- to 2-week CPF application period were significantly higher in pesticide applicators (6,437 μg/g creatinine) than in technicians (184 μg/g) and engineers (157 μg/g), both of whom are involved in supervising the application process. We observed a statistically significant inverse correlation between urinary TCPy and blood BuChE and AChE activities. The no-effect level (or inflection point) of the exposure–effect relationships has an average urinary TCPy level of 114 μg/g creatinine for BuChE and 3,161 μg/g creatinine for AChE. Our findings demonstrate a dose–effect relationship between urinary TCPy and both plasma BuChE and red blood cell AChE in humans exposed occupationally to CPF. These findings will contribute to future risk assessment efforts for CPF exposure.Keywords
This publication has 35 references indexed in Scilit:
- Repeated pesticide exposure among North Carolina migrant and seasonal farmworkersAmerican Journal of Industrial Medicine, 2010
- Cholinesterase Depression and Its Association with Pesticide Exposure across the Agricultural Season among Latino Farmworkers in North CarolinaEnvironmental Health Perspectives, 2010
- Chlorpyrifos exposures in Egyptian cotton field workersNeuroToxicology, 2010
- Blood Cholinesterases from Washington State Orchard WorkersBulletin of Environmental Contamination and Toxicology, 2009
- A Biomarker Validation Study of Prenatal Chlorpyrifos Exposure within an Inner-City Cohort during PregnancyEnvironmental Health Perspectives, 2009
- Cholinesterase inhibition in chlorpyrifos workers: Characterization of biomarkers of exposure and response in relation to urinary TCPyJournal of Exposure Science & Environmental Epidemiology, 2008
- Human Hepatic Cytochrome P450-Specific Metabolism of Parathion and ChlorpyrifosDrug Metabolism and Disposition, 2006
- Current issues in organophosphate toxicologyClinica Chimica Acta; International Journal of Clinical Chemistry, 2005
- Determinants of chlorpyrifos exposures and urinary 3,5,6-trichloro-2-pyridinol levels among termiticide applicatorsAnnals of Occupational Hygiene, 2001
- Common Mechanism of Toxicity: A Case Study of Organophosphorus Pesticides,Toxicological Sciences, 1998