The signals of FGFs on the neurogenesis of embryonic stem cells
Open Access
- 29 April 2010
- journal article
- Published by Springer Science and Business Media LLC in Journal of Biomedical Science
- Vol. 17 (1), 33
- https://doi.org/10.1186/1423-0127-17-33
Abstract
Neural induction is a complex process and the detailed mechanism of FGF-induced neurogenesis remains unclear. By using a serum-free neural induction method, we showed that FGF1 dose-dependently promoted the induction of Sox1/N-cadherin/nestin triple positive cells, which represent primitive neuroblasts, from mouse embryonic stem (ES) cells. We demonstrated that FGF1, FGF2, and FGF4, but not FGF8b, enhanced this neurogenesis. Especially, FGF-enhanced neurogenesis is not mediated through the rescue of the apoptosis or the enhancement of the proliferation of Sox1+ cells. We further indicated that the inactivation of c-Jun N-terminal kinase-1 (JNK-1) and extracellular signal-related kinase-2 (ERK-2), but not p38 mitogen-activated protein kinase (MAPK), inhibited the neural formation through the inhibition of ES differentiation, but not through the formation of endomesodermal cells. These lines of evidence delineated the roles of FGF downstream signals in the early neural differentiation of ES cells.Keywords
This publication has 33 references indexed in Scilit:
- Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cellsDevelopment, Growth & Differentiation, 2006
- Guiding embryonic stem cells towards differentiation: lessons from molecular embryologyCurrent Opinion in Genetics & Development, 2006
- Inhibited Neurogenesis in JNK1-Deficient Embryonic Stem CellsMolecular and Cellular Biology, 2005
- Neural induction requires BMP inhibition only as a late step, and involves signals other than FGF and Wnt antagonistsDevelopment, 2004
- DORSAL-VENTRAL PATTERNING AND NEURAL INDUCTION IN XENOPUS EMBRYOSAnnual Review of Cell and Developmental Biology, 2004
- In Vitro Development of Mouse Embryonic Stem Cells Lacking JNK/Stress-activated Protein Kinase-associated Protein 1 (JSAP1) Scaffold Protein Revealed Its Requirement during Early Embryonic NeurogenesisPublished by Elsevier BV ,2003
- Conversion of embryonic stem cells into neuroectodermal precursors in adherent monocultureNature Biotechnology, 2003
- Directed Differentiation of Embryonic Stem Cells into Motor NeuronsCell, 2002
- Defective Thymocyte Maturation in p44 MAP Kinase (Erk 1) Knockout MiceScience, 1999
- Site-Directed Mutagenesis and Molecular Modeling Identify a Crucial Amino Acid in Specifying the Heparin Affinity of FGF-1Biochemistry, 1999