Escherichia coliExpression, Purification, and Biological Activity of a Truncated Soluble CD4
- 1 April 1990
- journal article
- research article
- Published by Mary Ann Liebert Inc in AIDS Research and Human Retroviruses
- Vol. 6 (4), 465-479
- https://doi.org/10.1089/aid.1990.6.465
Abstract
A truncated molecule containing the N-terminal 183 amino acid residues of CD4 (sCD4-183) has been produced in Escherichia coli at high levels, using the trp promoter and an AT-rich ribosome binding site to direct expression in a pBR322-derived vector. A culture has been selected which allows large-scale fermentation and production of this material as an insoluble inclusion body protein. Procedures which solubilize, refold, and purify sCD4-183 have been developed. The purified sCD4-183 binds gp120 in solution and blocks human immunodeficiency virus (HIV) infection of human peripheral blood lymphocytes in vitro.Keywords
This publication has 31 references indexed in Scilit:
- A Rapid, Quantitative Bioassay Based on the Human Immunodeficiency VirusTrans-ActivatorAIDS Research and Human Retroviruses, 1989
- Substitution of murine for human CD4 residues identifies amino acids critical for HIV-gp120 bindingNature, 1988
- Internalization of the human immunodeficiency virus does not require the cytoplasmic domain of CD4Nature, 1988
- Blocking of HIV-1 Infectivity by a Soluble, Secreted Form of the CD4 AntigenScience, 1987
- Delineation of a region of the human immunodeficiency virus type 1 gp120 glycoprotein critical for interaction with the CD4 receptorCell, 1987
- Epitopes of the CD4 Antigen and HIV InfectionScience, 1986
- The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brainCell, 1986
- Role of the HTLV-III/LAV envelope in syncytium formation and cytopathicityNature, 1986
- Binding of HTLV-III/LAV to T4 + T Cells by a Complex of the 110K Viral Protein and the T4 MoleculeScience, 1986
- A set of synthetic oligodeoxyribonucleotide primers for DNA sequencing in the plasmid vector pBR322Gene, 1981