Ultrastructural and morphometric analysis of long-term peripheral nerve regeneration through silicone tubes
- 1 April 1988
- journal article
- Published by Springer Science and Business Media LLC in Journal of Neurocytology
- Vol. 17 (2), 161-172
- https://doi.org/10.1007/bf01674203
Abstract
Summary Light and electron microscopy were used to investigate long-term regeneration in peripheral nerves regenerating across a 10 mm gap through silicone tubes. Schwann cells and axons co-migrated behind an advancing front of fibroblasts, bridging the 10 mm gap between 28 and 35 days following nerve transection. Myelination of regenerated fibres started between 14 and 21 days after transection and occurred in a manner similar to that reported during development. Although these early events were successful in producing morphologically normal-appearing regenerated fibres, complete maturation of many of these fibres was never achieved. Axonal distortion by neurofilaments, axonal degeneration and secondary demyelination were seen at 56 days following nerve transection. These changes progressed in severity with time as more axons advanced through the distal stump towards their peripheral target. Since regeneration occurs in the absence of endoneurial tubes, and because constrictive forces act on the nerve during regeneration, we suggest that these extrinsic factors limit the successful advancement of axons through the distal stump to their target organ.Keywords
This publication has 39 references indexed in Scilit:
- Axons regenerated through silicone tube splicesExperimental Neurology, 1986
- Postnatal loss of axons in normal rat sciatic nerveJournal of Comparative Neurology, 1986
- Neurotoxic probes of the axonal cytoskeletonTrends in Neurosciences, 1983
- Temporal changes of neuronotrophic activities accumulating in vivo within nerve regeneration chambersExperimental Neurology, 1983
- Distortions of the nodes of Ranvier from axonal distension by filamentous masses in hexacarbon intoxicationJournal of Neurocytology, 1983
- The response of the (myelinating) Schwann cell population to multiple episodes of demyelinationJournal of Neurocytology, 1983
- Mouse sciatic nerve regeneration through semipermeable tubes: A quantitative modelJournal of Neuroscience Research, 1983
- Studies on the control of myelinogenesis. I. Myelination of regenerating axons after entry into a foreign unmyelinated nerveJournal of Neurocytology, 1975
- The role of Schwann cells in the development of human peripheral nervesJournal of Ultrastructure Research, 1965
- A banded structure in the connective tissue of nerveJournal of Ultrastructure Research, 1964