Increased Neutrophil-Platelet Adhesion in Patients With Unstable Angina
- 15 September 1996
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Circulation
- Vol. 94 (6), 1239-1246
- https://doi.org/10.1161/01.cir.94.6.1239
Abstract
BackgroundNeutrophil-platelet adhesion may occur as a consequence of platelet activation. The role of this heterotypic adhesion in ischemic disorders is poorly understood thus far.Methods and ResultsSystemic venous blood samples were taken from 25 patients with stable angina pectoris and 25 patients with unstable angina pectoris. Neutrophil activation and neutrophil-platelet adhesion were evaluated by two-color flow cytometry. Patients with unstable angina showed a significant increase in neutrophil-platelet adhesion compared with patients with stable angina (mean±SEM, 132.1±20.5 versus 29.8±4.7 anti-glycoprotein IIb/IIIa mean fluorescence intensity,P=.0001). Systemic neutrophil activation was found in patients with unstable angina compared with those with stable angina assessed by cell surface CD11b expression and shedding of L-selectin (115.6±10.3 versus 74.0±6.3 anti-CD11b mean fluorescence intensity,P=.002; 49.8±6.0 versus 72.1±4.0 anti–L-selectin mean fluorescence intensity,P=.006). Markers of neutrophil activation were related to the extent of neutrophil-platelet adhesion (CD11b:r=.5,P=.0005; L-selectin:r=.42,P=.012). In vitro studies revealed that binding of purified platelet membranes to control neutrophils caused a dose-dependent increase in CD11b surface expression, a decrease in surface L-selectin, and the release of superoxide anions.ConclusionsThus, this study demonstrates that increased neutrophil-platelet adhesion may contribute to neutrophil activation in unstable angina.Keywords
This publication has 27 references indexed in Scilit:
- Platelet-leukocyte aggregation during hemodialysisKidney International, 1994
- Expression of functional antigens on neutrophils: Effects of preparationJournal of Immunological Methods, 1992
- Effects of hemodialysis on platelet-derived thrombospondinKidney International, 1991
- Endothelial, platelet and leukocyte interactions in ischemic heart disease: Insights into potential mechanisms and their clinical relevanceJournal of the American College of Cardiology, 1990
- Neutrophil Mac-1 and MEL-14 Adhesion Proteins Inversely Regulated by Chemotactic FactorsScience, 1989
- Glycoprotein IV mediates thrombospondin-dependent platelet-monocyte and platelet-U937 cell adhesion.JCI Insight, 1989
- Stimulated mobilization of monocyte Mac-1 and p150,95 adhesion proteins from an intracellular vesicular compartment to the cell surface.JCI Insight, 1987
- Platelet Activation in Unstable Coronary DiseaseNew England Journal of Medicine, 1986
- Leukocytes and Ischemia-Induced Myocardial InjuryAnnual Review of Pharmacology and Toxicology, 1986
- Biological Defense Mechanisms. THE PRODUCTION BY LEUKOCYTES OF SUPEROXIDE, A POTENTIAL BACTERICIDAL AGENTJCI Insight, 1973