Evidence for a Hydrogenosomal-Type Anaerobic ATP Generation Pathway in Acanthamoeba castellanii
Open Access
- 27 September 2013
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (9), e69532
- https://doi.org/10.1371/journal.pone.0069532
Abstract
Diverse, distantly-related eukaryotic lineages have adapted to low-oxygen environments, and possess mitochondrion-related organelles that have lost the capacity to generate adenosine triphosphate (ATP) through oxidative phosphorylation. A subset of these organelles, hydrogenosomes, has acquired a set of characteristic ATP generation enzymes commonly found in anaerobic bacteria. The recipient of these enzymes could not have survived prior to their acquisition had it not still possessed the electron transport chain present in the ancestral mitochondrion. In the divergence of modern hydrogenosomes from mitochondria, a transitional organelle must therefore have existed that possessed both an electron transport chain and an anaerobic ATP generation pathway. Here, we report a modern analog of this organelle in the habitually aerobic opportunistic pathogen, Acanthamoeba castellanii. This organism possesses a complete set of enzymes comprising a hydrogenosome-like ATP generation pathway, each of which is predicted to be targeted to mitochondria. We have experimentally confirmed the mitochondrial localizations of key components of this pathway using tandem mass spectrometry. This evidence is the first supported by localization and proteome data of a mitochondrion possessing both an electron transport chain and hydrogenosome-like energy metabolism enzymes. Our work provides insight into the first steps that might have occurred in the course of the emergence of modern hydrogenosomes.This publication has 78 references indexed in Scilit:
- PhyloBayes 3: a Bayesian software package for phylogenetic reconstruction and molecular datingBioinformatics, 2009
- FastTree: Computing Large Minimum Evolution Trees with Profiles instead of a Distance MatrixMolecular Biology and Evolution, 2009
- Organelles in Blastocystis that Blur the Distinction between Mitochondria and HydrogenosomesCurrent Biology, 2008
- An Improved General Amino Acid Replacement MatrixMolecular Biology and Evolution, 2008
- Recent developments in the MAFFT multiple sequence alignment programBriefings in Bioinformatics, 2008
- Occurrence, Classification, and Biological Function of Hydrogenases: An OverviewChemical Reviews, 2007
- Locating proteins in the cell using TargetP, SignalP and related toolsNature Protocols, 2007
- TBestDB: a taxonomically broad database of expressed sequence tags (ESTs)Nucleic Acids Research, 2007
- RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed modelsBioinformatics, 2006
- An anaerobic mitochondrion that produces hydrogenNature, 2005