Effects of caffeine on mouse skeletal muscle power output during recovery from fatigue
Open Access
- 1 February 2004
- journal article
- Published by American Physiological Society in Journal of Applied Physiology
- Vol. 96 (2), 545-552
- https://doi.org/10.1152/japplphysiol.00696.2003
Abstract
The effects of 10 mM (high) and 70 μM (physiologically relevant) caffeine on force, work output, and power output of isolated mouse extensor digitorum longus (EDL) and soleus muscles were investigated in vitro during recovery from fatigue at 35°C. To monitor muscle performance during recovery from fatigue, we regularly subjected the muscle to a series of cyclical work loops. Force, work, and power output during shortening were significantly higher after treatment with 10 mM caffeine, probably as a result of increased Ca2+release from the sarcoplasmic reticulum. However, the work required to relengthen the muscle also increased in the presence of 10 mM caffeine. This was due to a slowing of relaxation and an increase in muscle stiffness. The combination of increased work output during shortening and increased work input during lengthening had different effects on the two muscles. Net power output of mouse soleus muscle decreased as a result of 10 mM caffeine exposure, whereas net power output of the EDL muscle showed a transient, significant increase. Treatment with 70 μM caffeine had no significant effect on force, work, or power output of EDL or soleus muscles, suggesting that the plasma concentrations found when caffeine is used to enhance performance in human athletes might not directly affect the contractile performance of fatigued skeletal muscle.Keywords
This publication has 15 references indexed in Scilit:
- Role of phosphate and calcium stores in muscle fatigueThe Journal of Physiology, 2001
- Interdependent effects of inorganic phosphate and creatine phosphate on sarcoplasmic reticulum Ca2+ regulation in mechanically skinned rat skeletal muscleThe Journal of Physiology, 2001
- Caffeine and ExerciseSports Medicine, 2001
- CaffeinePublished by Wiley ,2000
- The effects of caffeine on intracellular calcium, force and the rate of relaxation of mouse skeletal muscle.The Journal of Physiology, 1995
- Caffeine and Endurance PerformanceSports Medicine, 1994
- Changes of myoplasmic calcium concentration during fatigue in single mouse muscle fibers.The Journal of general physiology, 1991
- Slowing of relaxation during fatigue in single mouse muscle fibres.The Journal of Physiology, 1991
- Intracellular calcium and tension during fatigue in isolated single muscle fibres from Xenopus laevis.The Journal of Physiology, 1989
- Maximum tension and force‐velocity properties of fatigued, single Xenopus muscle fibres studied by caffeine and high K+.The Journal of Physiology, 1989