Cloning and Biochemical Characterization of a Class A β-Lactamase from Prevotella intermedia
- 1 August 2001
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 45 (8), 2386-9
- https://doi.org/10.1128/aac.45.8.2386-2389.2001
Abstract
The gene encoding a β-lactamase of Prevotella intermedia was cloned and sequenced. This gene, called cfxA2 , shared 98% identity with cfxA , the structural gene of a β-lactamase previously described in Bacteroides vulgatus . The deduced protein sequence had a K272E substitution. CfxA2 had the characteristics of class A, group 2e β-lactamases.Keywords
This publication has 21 references indexed in Scilit:
- A β-lactamase belonging to group 2e from oral clinical isolates ofPrevotella intermediaFEMS Microbiology Letters, 1998
- Evolution and Dissemination of β-Lactamases Accelerated by Generations of β-Lactam AntibioticsClinical Infectious Diseases, 1997
- Changes in β-lactam antibiotic susceptibility and β-lactamase production of clinical isolates of Bacleroides and Prevotella species over a 9 year periodJournal of Antimicrobial Chemotherapy, 1996
- Purification and characterization of a new beta-lactamase from Bacteroides uniformisAntimicrobial Agents and Chemotherapy, 1995
- β-Lactamase Production and Penicillin Susceptibility Among Different Ribotypes of Prevotella melaninogenica Simultaneously Colonizing the Oral CavityClinical Infectious Diseases, 1995
- Molecular and genetic analysis of the Bacteroides uniformis cephalosporinase gene, cblA, encoding the species-specific beta-lactamaseAntimicrobial Agents and Chemotherapy, 1994
- Sequence analysis of PER-1 extended-spectrum beta-lactamase from Pseudomonas aeruginosa and comparison with class A beta-lactamasesAntimicrobial Agents and Chemotherapy, 1994
- Genetic and biochemical analysis of a novel Ambler class A beta-lactamase responsible for cefoxitin resistance in Bacteroides speciesAntimicrobial Agents and Chemotherapy, 1993
- NOTES: Prevotella, a New Genus To Include Bacteroides melaninogenicus and Related Species Formerly Classified in the Genus BacteroidesInternational Journal of Systematic and Evolutionary Microbiology, 1990
- Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferaseGene, 1988