The Crl protein activates cryptic genes for curli formation and fibronectin binding in Escherichia coli HB101
- 1 September 1992
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 6 (17), 2443-2452
- https://doi.org/10.1111/j.1365-2958.1992.tb01420.x
Abstract
Curli are thin, coiled, temperature-regulated fibres on fibronectin-binding Escherichia coli. The subunit protein of curli was highly homologous at its amino terminus to SEF-17, the subunit protein of thin, aggregative fimbriae of Salmonella enteritidis 27655 strain 3b, suggesting that these fibres form a novel class of surface organelles on enterobacteria. E. coli HB101 is non-curliated and unable to bind soluble, iodinated fibronectin. The phenotypically cryptic curlin subunit gene, csgA, in HB101 is transcriptionally activated by expressing the cytoplasmic Crl on a multicopy plasmid. Transcriptional activation of csgA by Crl was observed after growth at 26 degrees C but not at 37 degrees C, even though crl transcription was not thermoregulated. A deletion of the 39 carboxy-terminal residues abolished Crl activity, whereas a deletion of 10 residues at the C-terminus did not, implying that a region between residue 93 and 122 in the 132-amino-acid-residue large Crl protein is required for activating curli expression in E. coli HB101. crl is a normal housekeeping gene in E. coli and it is suggested that its gene product may either be a DNA-binding protein affecting chromatin structure as has been suggested for histone-like protein H1 or interact with specific regulatory protein(s) controlling transcription of genes required for curli formation and fibronectin binding.Keywords
This publication has 33 references indexed in Scilit:
- CHAPERONE-ASSISTED ASSEMBLY AND MOLECULAR ARCHITECTURE OF ADHESIVE PILIAnnual Review of Microbiology, 1991
- The molecular basis of carbon‐starvation‐induced general resistance in Escherichia coliMolecular Microbiology, 1991
- Histone-like protein H1 (H-NS), DNA supercoiling, and gene expression in bacteriaCell, 1990
- Upstream activating sequences that are shared by two divergently transcribed operons mediate cAMP‐CRP regulation of pilus‐adhesin in Escherichia coliMolecular Microbiology, 1989
- Differentially expressed trmD ribosomal protein operon of Escherichia coli is transcribed as a single polycistronic mRNA speciesJournal of Molecular Biology, 1989
- Characterization of the neisserial lipid-modified azurin bearing the H.8 epitopeMolecular Microbiology, 1989
- Complete nucleotide sequence of phoE, the structural gene for the phosphate limitation inducible outer membrane pore protein of Escherichia coli K12Journal of Molecular Biology, 1983
- Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencingJournal of Molecular Biology, 1980
- A complementation analysis of the restriction and modification of DNA in Escherichia coliJournal of Molecular Biology, 1969
- The Preferential Synthesis of -Galactosidase in Escherichia coliJournal of General Microbiology, 1955