Genetic Polymorphism of Aspergillus fumigatus in Clinical Samples from Patients with Invasive Aspergillosis: Investigation Using Multiple Typing Methods
Open Access
- 1 May 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 39 (5), 1731-1737
- https://doi.org/10.1128/jcm.39.5.1731-1737.2001
Abstract
The genotypes of 52 strains of Aspergillus fumigatus isolated from 12 patients with invasive aspergillosis were investigated using three typing methods (random amplified polymorphic DNA, sequence-specific DNA polymorphism, and microsatellite polymorphism) combined with multilocus enzyme electrophoresis. Isolates were from patients hospitalized in three different geographic areas (Lyon, France; Grenoble, France; and Milan, Italy). In each case, the genetic polymorphism of several colonies (two to five) within the first respiratory clinical sample was studied. For the 52 isolates tested, random amplified polymorphic DNA identified 8 different genotypes, sequence-specific DNA polymorphism identified 9 different types, and microsatellite polymorphism identified 14 types. A combination of these results with multilocus enzyme electrophoresis study identified 25 different types within the sample studied. We identified 3 patients (of the 12 studied) who carried a single genotype; 6 patients were infected by two genotypes, 1 patient had four genotypes, while the last patient had five. A combination of typing methods provided better discrimination than the use of a single method. Typing methods revealed a population structure within each geographical site, suggesting that the epidemiology of A. fumigatus should be considered separately for each of these geographic areas. This study demonstrates the usefulness of combining several typing methods in reaching an understanding of the epidemiology of A. fumigatus and clarifies whether it is sufficient to type one isolate from each specimen to determine the strain involved in invasive aspergillosis.Keywords
This publication has 29 references indexed in Scilit:
- Combination of three typing methods for the molecular epidemiology of Aspergillus fumigatus infectionsJournal of Medical Microbiology, 1999
- Invasive AspergillosisClinical Infectious Diseases, 1998
- Diagnostic and Therapeutic Strategies for Invasive AspergillosisSeminars in Respiratory and Critical Care Medicine, 1997
- Afut1, a retrotransposon-like element from Aspergillus fumigatusNucleic Acids Research, 1996
- Simultaneous carriage ofCandida albicansstrains from HIV-infected patients with oral candidiasis: multilocus enzyme electrophoresis analysisFEMS Microbiology Letters, 1996
- Use of phenotypic and genotypic fingerprinting methods in the strain identification of Aspergillus fumigatusMycoses, 1995
- Epidemiology and control of nosocomial fungal infectionsCurrent Opinion in Infectious Diseases, 1995
- Molecular typing of Aspergillus speciesJournal of Hospital Infection, 1995
- DNA typing of epidemiologically-related isolates ofAspergillus fumigatusEpidemiology and Infection, 1995
- The Statistics of Natural Selection on Animal PopulationsJournal of Animal Ecology, 1987