S1 gene sequence heterogeneity of a pathogenic infectious bronchitis virus strain and its embryo-passaged, attenuated derivatives
- 11 May 2007
- journal article
- research article
- Published by Taylor & Francis Ltd in Avian Pathology
- Vol. 36 (3), 231-234
- https://doi.org/10.1080/03079450701338730
Abstract
Infectious bronchitis virus CK/CH/LDL/97I was attenuated by serial passage in chicken embryos. Virus of passage 115 was attenuated as determined by clinical response to inoculation to 15-day-old specific pathogen free chickens. The vaccination-challenge test showed that the attenuated passage 115 virus could afford protection against the homologous pathogenic virus, passage 5, by the clinical response. Based on the sequence analysis and comparison of the S1 gene, both the pathogenic and attenuated CK/CH/LDL/97I viruses were populations that each included at least two subpopulations. Also, from analysis of the amino acid and nucleotide sequences of S1 gene, we speculate that recombination between the minor and dominant subpopulations plus accumulation of mutations in the S1 region of pathogenic passage 5 might lead to the formation of the embryo-passaged, attenuated passage 115.Keywords
This publication has 16 references indexed in Scilit:
- Infectious bronchitis virus: S1 gene characteristics of vaccines used in China and efficacy of vaccination against heterologous strains from ChinaAvian Pathology, 2006
- Genetic diversity of avian infectious bronchitis coronavirus strains isolated in China between 1995 and 2004Archiv für die gesamte Virusforschung, 2006
- Selection of and recombination between minor variants lead to the adaptation of an avian coronavirus to primate cellsBiochemical and Biophysical Research Communications, 2005
- Variation in the spike protein of the 793/B type of infectious bronchitis virus, in the field and during alternate passage in chickens and embryonated eggsAvian Pathology, 2005
- A new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and non-vaccinated flocks in ChinaAvian Pathology, 2004
- Protection of chickens against renal damage caused by a nephropathogenic infectious bronchitis virusAvian Pathology, 2001
- Transmission of infectious bronchitis virus within vaccinated and unvaccinated groups of chickensAvian Pathology, 1998
- Molecular analysis of the 793/B serotype of infectious bronchitis virus in Great BritainAvian Pathology, 1997
- Location of the amino acid differences in the S1 spike glycoprotein subunit of closely related serotypes of infectious bronchitis virusAvian Pathology, 1992
- Coronavirus IBV: Removal of Spike Glycopolypeptide S1 by Urea Abolishes Infectivity and Haemagglutination but Not Attachment to CellsJournal of General Virology, 1986