Calcium/calmodulin-dependent kinase IV contributes to translation-dependent early synaptic potentiation in the anterior cingulate cortex of adult mice
Open Access
- 16 September 2010
- journal article
- research article
- Published by Springer Science and Business Media LLC in Molecular Brain
- Vol. 3 (1), 27
- https://doi.org/10.1186/1756-6606-3-27
Abstract
Calcium/calmodulin-dependent kinase IV (CaMKIV) phosphorylates the major transcription factor, cyclic AMP-responsive element binding protein (CREB), which plays key roles in synaptic plasticity and memory consolidation. Our previous study showed that long-term potentiation (LTP) in the anterior cingulate cortex (ACC) was significantly enhanced in transgenic mice overexpressing CaMKIV. Considering that the CaMKIV-CREB pathway plays a central role in the protein synthesis-dependent LTP, it is possible that upregulation of CaMKIV contributes to enhancement of LTP by promoting protein synthesis. To test this possibility, we examined the effects of transcription and translation inhibitors on synaptic potentiation induced by pairing of synaptic activity with postsynaptic depolarization (paired training) in ACC pyramidal neurons of wild-type and CaMKIV transgenic mice. We found that synaptic potentiation induced by paired training was partially inhibited by transcription or translation inhibitors both in wild-type and CaMKIV transgenic mice; the extent of inhibition was markedly larger in the CaMKIV transgenic mice than in the wild-type mice. Biochemical and immunohistochemical studies revealed that CaMKIV was distributed in the membrane, cytosol and nucleus of ACC neurons. Our results reveal in the first time a transcription- and translation-dependent component of early synaptic LTP in adult ACC synapses, and demonstrate that CaMKIV enhances early synaptic potentiation by activating new protein synthesis.This publication has 63 references indexed in Scilit:
- CaMKIV over-expression boosts cortical 4-7 Hz oscillations during learning and 1-4 Hz delta oscillations during sleepMolecular Brain, 2010
- Characterization of intracortical synaptic connections in the mouse anterior cingulate cortex using dual patch clamp recordingMolecular Brain, 2009
- Sexual attraction enhances glutamate transmission in mammalian anterior cingulate cortexMolecular Brain, 2009
- Plasticity of NMDA receptor NR2B subunit in memory and chronic painMolecular Brain, 2009
- Characterization of Intrinsic Properties of Cingulate Pyramidal Neurons in Adult Mice after Nerve InjuryMolecular Pain, 2009
- Induction- and conditioning-protocol dependent involvement of NR2B-containing NMDA receptors in synaptic potentiation and contextual fear memory in the hippocampal CA1 region of ratsMolecular Brain, 2008
- Cingulate NMDA NR2B receptors contribute to morphine-induced analgesic toleranceMolecular Brain, 2008
- Requirement of Extracellular Signal-Regulated Kinase/Mitogen-Activated Protein Kinase for Long-Term Potentiation in Adult Mouse Anterior Cingulate CortexMolecular Pain, 2007
- Transcriptional regulation by the phosphorylation-dependent factor CREBNature Reviews Molecular Cell Biology, 2001
- Control of Memory Formation Through Regulated Expression of a CaMKII TransgeneScience, 1996