Fast analysis of isobaric grape anthocyanins by Chip‐liquid chromatography/mass spectrometry
- 10 August 2009
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 23 (18), 2891-2896
- https://doi.org/10.1002/rcm.4199
Abstract
In a previous work, direct‐infusion electrospray ionization ion trap tandem mass spectrometry (ESI‐IT‐MS/MS) was applied to the study of anthocyanins in extracts from the skins of Clinton grapes, a non‐Vitis vinifera red grape variety qualitatively and quantitatively rich in anthocyanins. A good characterization of anthocyaninins was obtained, but it was impossible to differentiate some compounds with the same nominal mass but with different elemental composition. In this work, the capabilities of quadrupole time‐of‐flight mass spectrometry (QTOF‐MS) coupled with Chip‐liquid chromatography (LC‐Chip) were applied to the study of Clinton anthocyanins and this method provided the complete sample anthocyanin fingerprint in less than 5 min. Multi‐stage mass spectrometry (MSn; n >2) was not necessary to identify isobaric compounds, nor were deuterium‐exchange experiments necessary to distinguish between compounds containing the same aglycone. The fast separation bypasses the problem of petunidin‐3‐O‐(6‐O‐acetyl)monoglucoside and delphinidin‐3,5‐O‐diglucoside quantification, present in the direct‐infusion ESI‐ITMS approach, due to overlapping with matrix interferences. Copyright © 2009 John Wiley & Sons, Ltd.Keywords
This publication has 9 references indexed in Scilit:
- Mass spectrometry in the study of anthocyanins and their derivatives: differentiation of Vitis vinifera and hybrid grapes by liquid chromatography/electrospray ionization mass spectrometry and tandem mass spectrometryJournal of Mass Spectrometry, 2004
- Mass Spectrometric Evidence for the Existence of Oligomeric Anthocyanins in Grape SkinsJournal of Agricultural and Food Chemistry, 2004
- Characterization of Anthocyanins in Grape Juices by Ion Trap Liquid Chromatography−Mass SpectrometryJournal of Agricultural and Food Chemistry, 2003
- Isolation and Structures of Oligomeric Wine Pigments by Bisulfite-Mediated Ion-Exchange ChromatographyJournal of Agricultural and Food Chemistry, 2001
- Application of Electrospray Ionization Mass Spectrometry to the Study of Grape AnthocyaninsAmerican Journal of Enology and Viticulture, 2000
- Electrospray and Tandem Mass Spectroscopy As Tools for Anthocyanin CharacterizationJournal of Agricultural and Food Chemistry, 1999
- Identification of anthocyanin derivatives in grape skin extracts and red wines by liquid chromatography with diode array and mass spectrometric detectionJournal of Chromatography A, 1999
- Comparison of Several Procedures Used for the Extraction of Anthocyanins from Red GrapesJournal of Agricultural and Food Chemistry, 1998
- HPLC/MS Application to Anthocyanins of Vitis vinifera L.Journal of Agricultural and Food Chemistry, 1995