Comparison of conventional smear and liquid-based cytology preparation in diagnosis of lung cancer by bronchial wash and transbronchial needle aspiration
Open Access
- 1 January 2018
- journal article
- research article
- Published by Medknow in Journal of Cytology
- Vol. 35 (2), 94-98
- https://doi.org/10.4103/joc.joc_248_16
Abstract
Introduction: Liquid-based cytology (LBC), initially developed for screening gynecologic specimens, is now being used in nongynecologic aspiration and exfoliative specimens. In this study, the diagnostic yield and utility of thin-prep (TP) was compared with conventional preparations to ascertain its utility in improving the diagnosis of respiratory lesions. Materials and Methods: Bronchial washings (BW) and transbronchial needle aspirates (TBNA) (bronchoscopy/endobronchial ultrasound-guided) from 70 consecutive patients of mediastinal masses and endo/peribronchial growths were included. The diagnostic yields of both conventional smears and thin-prep were compared. Immunocytochemistry (ICC) was performed on direct/cytospin smears of TBNA/BW and TP slides when the tumor could not be subtyped by morphology. Histopathologic correlation was done. Results: Although well-preserved morphological features and cleaner background in TP allowed accurate diagnosis of malignancies, diagnostic yield was comparable to conventional preparations. Immunocytochemistry was successfully employed on TP smears which helped in accurate subtyping of the tumors. Few shortcomings of TP smears were uneven distribution of cells, thick cell clusters, and inadequate cellularity. Conclusion: Liquid-based TP preparation is an effective diagnostic tool for respiratory tract cytology, however, results are comparable to conventional smears.Keywords
This publication has 26 references indexed in Scilit:
- c-Myb Overexpression in Cytology Smears of Tracheobronchial and Pulmonary Adenoid Cystic CarcinomasActa Cytologica, 2016
- Introduction to The 2015 World Health Organization Classification of Tumors of the Lung, Pleura, Thymus, and HeartJournal of Thoracic Oncology, 2015
- Liquid-Based Cytology Preparation Can Improve Cytological Assessment of Endobronchial Ultrasound-Guided Transbronchial Needle AspirationActa Cytologica, 2015
- Cytomorphology of neuroendocrine tumours of the gallbladderCytopathology, 2015
- Initial Experience With Endobronchial Ultrasound-guided Transbronchial Needle Aspiration (EBUS-TBNA) From a Tuberculosis Endemic PopulationJournal of Bronchology & Interventional Pulmonology, 2014
- EBUS-TBNA Provides Highest RNA Yield for Multiple Biomarker Testing from Routinely Obtained Small Biopsies in Non-Small Cell Lung Cancer Patients - A Comparative Study of Three Different Minimal Invasive Sampling MethodsPLOS ONE, 2013
- Suitability of Endobronchial Ultrasound-guided Transbronchial Needle Aspiration Specimens for Subtyping and Genotyping of Non–Small Cell Lung CancerAmerican Journal of Respiratory and Critical Care Medicine, 2012
- Liquid-Based vs. Conventional Cytology on Respiratory MaterialActa Cytologica, 2009
- The role of ThinPrep cytology in the investigation of lung tumorsSurgical Oncology, 2006
- Immunocytochemistry on the Thinprep® processorDiagnostic Cytopathology, 1997