A method for volume selective nuclear magnetic resonance spectroscopy has been developed and implemented on an 1.5 T whole body imager for in vivo investigations. Four single experiments produce different magnetizations in the same slice, and a special subtract scheme yields the signal of only the volume of interest, which is accurately defined. The resolution of the spectra and the stability of the method have been verified with a water phantom containing acetone, ethanol, methanol, and oil vessels.