Bone marrow‐derived mesenchymal stem cells express the pericyte marker 3G5 in culture and show enhanced chondrogenesis in hypoxic conditions
Open Access
- 14 April 2010
- journal article
- research article
- Published by Wiley in Journal of Orthopaedic Research
- Vol. 28 (6), 834-840
- https://doi.org/10.1002/jor.21043
Abstract
Bone marrow-derived mesenchymal stem cells are a potential source of cells for the repair of articular cartilage defects. Hypoxia has been shown to improve chondrogenesis in some cells. In this study, bone marrow-derived stem cells were characterized and the effects of hypoxia on chondrogenesis investigated. Adherent bone marrow colony-forming cells were characterized for stem cell surface epitopes, and then cultured as cell aggregates in chondrogenic medium under normoxic (20% oxygen) or hypoxic (5% oxygen) conditions. The cells stained strongly for markers of adult mesenchymal stem cells, and a high number of cells were also positive for the pericyte marker 3G5. The cells showed a chondrogenic response in cell aggregate cultures and, in lowered oxygen, there was increased matrix accumulation of proteoglycan, but less cell proliferation. In hypoxia, there was increased expression of key transcription factor SOX6, and of collagens II and XI, and aggrecan. Pericytes are a candidate stem cell in many tissue, and our results show that bone marrow-derived mesenchymal stem cells express the pericyte marker 3G5. The response to chondrogenic culture in these cells was enhanced by lowered oxygen tension. This has important implications for tissue engineering applications of bone marrow-derived stem cells. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:834–840, 2010Keywords
This publication has 43 references indexed in Scilit:
- Skeletal stem cells: Phenotype, biology and environmental niches informing tissue regenerationMolecular and Cellular Endocrinology, 2008
- Hypoxic conditions increase hypoxia-inducible transcription factor 2α and enhance chondrogenesis in stem cells from the infrapatellar fat pad of osteoarthritis patientsArthritis Research & Therapy, 2007
- Differential Regulation of the Transcriptional Activities of Hypoxia-Inducible Factor 1 Alpha (HIF-1α) and HIF-2α in Stem CellsMolecular and Cellular Biology, 2006
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Effect of Oxygen Tension and Alginate Encapsulation on Restoration of the Differentiated Phenotype of Passaged ChondrocytesTissue Engineering, 2001
- Cultivation of rat marrow‐derived mesenchymal stem cells in reduced oxygen tension: Effects on in vitro and in vivo osteochondrogenesisJournal of Cellular Physiology, 2001
- Multilineage Cells from Human Adipose Tissue: Implications for Cell-Based TherapiesTissue Engineering, 2001
- Mechanisms of chondrocyte apoptosisOsteoarthritis and Cartilage, 1999
- Mesenchymal stem cellsJournal of Orthopaedic Research, 1991
- Bone marrow circulation after osteotomy: Blood flow, pO2, pCO2, and pressure studied in dogsActa Orthopaedica, 1985