Estimation of monoamine oxidase concentrations in soluble and membrane-bound preparations by inhibitor binding
- 1 January 1994
- book chapter
- conference paper
- Published by Springer Science and Business Media LLC
- Vol. 41, 47-53
- https://doi.org/10.1007/978-3-7091-9324-2_6
Abstract
A modification of the [3H]-pargyline labelling technique is presented for determining the active-site concentration of monoamine oxidase in soluble preparations. Kinetic considerations show that the rate of reaction of MAO-A with low concentrations of free pargyline will be very much slower than that of MAO-B. Failure to use adequate reaction times for the concentration of pargyline added can lead to gross underestimation of the quantity of MAO-A present.Keywords
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