Comparison of immunological reactivity of polygalacturonases from different fungi
- 1 January 1988
- journal article
- research article
- Published by Informa UK Limited in Giornale botanico italiano
- Vol. 122 (1), 1-6
- https://doi.org/10.1080/11263508809429387
Abstract
Homogeneous endo-polygalacturonases (PGs) from Aspergillus niger and Fusarium moniliforme were used to prepare rabbit antisera. IgG were purified from antisera by Protein A-sepharose chromatography. Purified IgG were tested against endopolygalacturonases from Sclerotium rolfsii, Sclerotinia sclerotiorum, Rhizoctonia solani, Rhizoctonia fragariae and Fusarium oxysporum. Double immunodiffusion tests, ELISA tests and Western blottings showed that all the enzymes react with both antisera suggesting that a basic PG structure is mantained among the different fungi.Keywords
This publication has 12 references indexed in Scilit:
- Elicitation of Necrosis in Vigna unguiculata Walp. by Homogeneous Aspergillus niger Endo-Polygalacturonase and by α-d-Galacturonate OligomersPlant Physiology, 1987
- Molecular Evolution of Fungal PolygalacturonasePublished by Springer Science and Business Media LLC ,1986
- Endopolygalacturonase zymograms of Fusarium speciesTransactions of the British Mycological Society, 1981
- Host-Pathogen InteractionsPlant Physiology, 1981
- [31] Use of protein A—bearing staphylococci for the immunoprecipitation and isolation of antigens from cellsMethods in enzymology, 1981
- Polygalacturonase from Rhizoctonia fragariae: further characterization of two isoenzymes and their action towards strawberry tissuePhysiological Plant Pathology, 1978
- Endopolygalacturonase from Rhizoctonia fragariae Purification and characterization of two isoenzymesBiochimica et Biophysica Acta (BBA) - Enzymology, 1977
- Degradation of Plant Cell Walls and Membranes by Microbial EnzymesPublished by Springer Science and Business Media LLC ,1976
- Host-pathogen interactions IV. Studies on the polysaccharide-degrading enzymes secreted by Fusarium oxysporum f. sp. lycopersiciPhysiological Plant Pathology, 1972
- Polysaccharide-degrading Enzymes are Unable to Attack Plant Cell Walls without Prior Action by a “Wall-modifying Enzyme”Plant Physiology, 1970