Mass spectrometry tools and workflows for revealing microbial chemistry
- 27 April 2015
- journal article
- review article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 140 (15), 4949-4966
- https://doi.org/10.1039/c5an00171d
Abstract
Since the time Van Leeuwenhoek was able to observe microbes through a microscope, an innovation that led to the birth of the field of microbiology, we have aimed to understand how microorganisms function, interact and communicate. The exciting progress in the development of analytical technologies and workflows has demonstrated that mass spectrometry is a very powerful technique for the interrogation of microbiology at the molecular level. In this review, we aim to highlight the available and emerging tools in mass spectrometry for microbial analysis by overviewing the methods and workflow advances for taxonomic identification, microbial interaction, dereplication and drug discovery. We emphasize their potential for future development and point out unsolved problems and future directions that would aid in the analysis of the chemistry produced by microbes.Keywords
This publication has 186 references indexed in Scilit:
- Dual Analysis for Mycobacteria and Propionibacteria in Sarcoidosis BALJournal of Clinical Immunology, 2012
- Lessons from the Past and Charting the Future of Marine Natural Products Drug Discovery and Chemical BiologyCell Chemical Biology, 2012
- Imaging Mass Spectrometry and Genome Mining via Short Sequence Tagging Identified the Anti-Infective Agent Arylomycin in Streptomyces roseosporusJournal of the American Chemical Society, 2011
- Antibiotics as Signal MoleculesChemical Reviews, 2011
- Connecting Chemotypes and Phenotypes of Cultured Marine Microbial Assemblages by Imaging Mass SpectrometryAngewandte Chemie, 2011
- Integrating ‘-omics’ and natural product discovery platforms to investigate metabolic exchange in microbiomesCurrent Opinion in Chemical Biology, 2011
- The evolving field of imaging mass spectrometry and its impact on future biological researchJournal of Mass Spectrometry, 2011
- Natural Products Version 2.0: Connecting Genes to MoleculesJournal of the American Chemical Society, 2010
- Scanning Mass Spectrometry Probe: A Scanning Probe Electrospray Ion Source for Imaging Mass Spectrometry of Submerged Interfaces and Transient Events in SolutionAnalytical Chemistry, 2009
- Palmyramide A, a Cyclic Depsipeptide from a Palmyra Atoll Collection of the Marine CyanobacteriumLyngbya majusculaJournal of Natural Products, 2009