Epithelial Cells Lining Salivary Gland Ducts Are Early Target Cells of Severe Acute Respiratory Syndrome Coronavirus Infection in the Upper Respiratory Tracts of Rhesus Macaques
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- 15 April 2011
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 85 (8), 4025-4030
- https://doi.org/10.1128/jvi.02292-10
Abstract
The shedding of severe acute respiratory syndrome coronavirus (SARS-CoV) into saliva droplets plays a critical role in viral transmission. The source of high viral loads in saliva, however, remains elusive. Here we investigate the early target cells of infection in the entire array of respiratory tissues in Chinese macaques after intranasal inoculations with a single-cycle pseudotyped virus and a pathogenic SARS-CoV. We found that angiotensin-converting enzyme 2-positive (ACE2 + ) cells were widely distributed in the upper respiratory tract, and ACE2 + epithelial cells lining salivary gland ducts were the early target cells productively infected. Our findings also have implications for SARS-CoV early diagnosis and prevention.Keywords
This publication has 45 references indexed in Scilit:
- Exacerbated Innate Host Response to SARS-CoV in Aged Non-Human PrimatesPLoS Pathogens, 2010
- Early Upregulation of Acute Respiratory Distress Syndrome-Associated Cytokines Promotes Lethal Disease in an Aged-Mouse Model of Severe Acute Respiratory Syndrome Coronavirus InfectionJournal of Virology, 2009
- MyD88 Is Required for Protection from Lethal Infection with a Mouse-Adapted SARS-CoVPLoS Pathogens, 2008
- The SARS-CoV ferret model in an infection–challenge studyVirology, 2008
- A novel subset of putative stem/progenitor CD34+Oct-4+ cells is the major target for SARS coronavirus in human lungThe Journal of Experimental Medicine, 2007
- Natural Mutations in the Receptor Binding Domain of Spike Glycoprotein Determine the Reactivity of Cross-Neutralization between Palm Civet Coronavirus and Severe Acute Respiratory Syndrome CoronavirusJournal of Virology, 2007
- Cynomolgus Macaque as an Animal Model for Severe Acute Respiratory SyndromePLoS Medicine, 2006
- Time Course and Cellular Localization of SARS-CoV Nucleoprotein and RNA in Lungs from Fatal Cases of SARSPLoS Medicine, 2006
- A DNA vaccine induces SARS coronavirus neutralization and protective immunity in miceNature, 2004
- The cytoplasmic body component TRIM5α restricts HIV-1 infection in Old World monkeysNature, 2004