A method for removal of trace iron contamination from biological buffers
- 20 April 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 214 (2), 362-364
- https://doi.org/10.1016/0014-5793(87)80088-1
Abstract
Laboratory chemicals and reagents normally contain trace amounts of iron salts sufficient to catalyse free radical reactions. This iron contamination can be removed from buffers and reagents using a dialysis sac containing a high-affinity iron-binding protein like conalbumin or transferrin without altering the pH value of the fluid.Keywords
This publication has 10 references indexed in Scilit:
- Oxygen free radicals and iron in relation to biology and medicine: Some problems and conceptsArchives of Biochemistry and Biophysics, 1986
- Iron Complexes and Their Reactivity in the Bleomycin Assay for Radical-Promoting Loosely-Bound IronFree Radical Research Communications, 1986
- Cytotoxicity from Coupled Redox Cycling of Autoxidizing Xenobiotics and Metals: A Selective Critical Review and Commentary on Work‐in‐ProgressIsrael Journal of Chemistry, 1984
- Superoxide-dependent formation of hydroxyl radicals in the presence of iron salts. Detection of ‘free’ iron in biological systems by using bleomycin-dependent degradation of DNABiochemical Journal, 1981
- Inhibition of lipid peroxidation by the iron-binding protein lactoferrinBiochemical Journal, 1981
- The role of superoxide and hydroxyl radicals in the degradation of hyaluronic acid induced by metal ions and by ascorbic acidJournal of Inorganic Biochemistry, 1981
- The Effect of pH upon Human Transferrin: Selective Labelling of the Two Iron‐binding SitesBritish Journal of Haematology, 1976
- Reduced Triphosphopyridine Nucleotide Oxidase-catalyzed Alterations of Membrane PhospholipidsPublished by Elsevier BV ,1971
- ADP-activated lipid peroxidation coupled to the TPNH oxidase system of microsomesBiochemical and Biophysical Research Communications, 1963
- The Isolation of a Red Protein from Milk2Journal of the American Chemical Society, 1960