Altered microRNA expression in patients with non-obstructive azoospermia
Open Access
- 11 February 2009
- journal article
- research article
- Published by Springer Science and Business Media LLC in Reproductive Biology and Endocrinology
- Vol. 7 (1), 1-10
- https://doi.org/10.1186/1477-7827-7-13
Abstract
Background MicroRNAs (miRNAs), a class of small non-coding RNA molecules, are indicated to play essential roles in spermatogenesis. However, little is known about the expression patterns or function of miRNAs in human testes involved in infertility. Methods In this study, the miRNA expression profiles of testes of patients with non-obstructive azoospermia (NOA) and normal controls were performed by using microarray technologies. Results Altered microRNA expression in NOA patients was found, with 154 differentially down-regulated and 19 up-regulated miRNAs. These findings have been confirmed by real-time reverse transcription-polymerase chain reaction (RT-PCR) assays on select miRNAs, including miR-302a, miR-491-3p, miR-520d-3p and miR-383. Several down-regulated miRNA clusters in patients with NOA were identified, such as the oncogenic potential of the mir-17-92 cluster and mir-371,2,3 cluster. Conclusion This is the first report that the expression of miRNAs is altered in testicular tissues of patients with NOA, suggesting a role of miRNAs in regulating spermatogenesis in human males.Keywords
This publication has 31 references indexed in Scilit:
- Genome-Wide Expression of Azoospermia Testes Demonstrates a Specific Profile and Implicates ART3 in Genetic SusceptibilityPLoS Genetics, 2008
- A single Hox locus in Drosophila produces functional microRNAs from opposite DNA strandsGenes & Development, 2008
- Cloning and expression profiling of testis-expressed microRNAsDevelopmental Biology, 2007
- Abnormal progression through meiosis in men with nonobstructive azoospermiaFertility and Sterility, 2007
- The chromatoid body: a germ-cell-specific RNA-processing centreNature Reviews Molecular Cell Biology, 2007
- Mouse MAELSTROM: the link between meiotic silencing of unsynapsed chromatin and microRNA pathway?Human Molecular Genetics, 2006
- The chromatoid body of male germ cells: Similarity with processing bodies and presence of Dicer and microRNA pathway componentsProceedings of the National Academy of Sciences of the United States of America, 2006
- MicroRNA Mirn122a Reduces Expression of the Posttranscriptionally Regulated Germ Cell Transition Protein 2 (Tnp2) Messenger RNA (mRNA) by mRNA Cleavage1Biology of Reproduction, 2005
- Variation in meiotic recombination frequencies among human malesHuman Genetics, 2004
- Meiotic defects in a man with non-obstructive azoospermia: Case reportHuman Reproduction, 2004