Production of biologically active salmon calcitonin in the milk of transgenic rabbits
- 1 July 1998
- journal article
- Published by Springer Science and Business Media LLC in Nature Biotechnology
- Vol. 16 (7), 647-651
- https://doi.org/10.1038/nbt0798-647
Abstract
Salmon calcitonin (sCT) is an example of one of the many bioactive peptides that require amidation of the carboxy terminus for full potency. We describe a method for the production of amidated sCT in the mammary gland of transgenic rabbits. Expression of a fusion protein comprising human alpha lactalbumin joined by an enterokinase cleavable linker to sCT was directed to the mammary gland under the control of the ovine beta lactoglobulin promoter. C-terminal amidation in vivo was achieved by extending the sCT by a single glycine residue that provides a substrate for endogenous amidating activity in the mammary gland. Full characterization of the released sCT demonstrated it to be equivalent to synthetic standard in terms of structure, purity, and potency.Keywords
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