Shear stress enhances human endothelial cell wound closure in vitro
Open Access
- 1 July 2000
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Heart and Circulatory Physiology
- Vol. 279 (1), H293-H302
- https://doi.org/10.1152/ajpheart.2000.279.1.h293
Abstract
Repair of the endothelium occurs in the presence of continued blood flow, yet the mechanisms by which shear forces affect endothelial wound closure remain elusive. Therefore, we tested the hypothesis that shear stress enhances endothelial cell wound closure. Human umbilical vein endothelial cells (HUVEC) or human coronary artery endothelial cells (HCAEC) were cultured on type I collagen-coated coverslips. Cell monolayers were sheared for 18 h in a parallel-plate flow chamber at 12 dyn/cm2 to attain cellular alignment and then wounded by scraping with a metal spatula. Subsequently, the monolayers were exposed to a laminar shear stress of 3, 12, or 20 dyn/cm2 under shear-wound-shear (S-W-sH) or shear-wound-static (S-W-sT) conditions for 6 h. Wound closure was measured as a percentage of original wound width. Cell area, centroid-to-centroid distance, and cell velocity were also measured. HUVEC wounds in the S-W-sH group exposed to 3, 12, or 20 dyn/cm2 closed to 21, 39, or 50%, respectively, compared with only 59% in the S-W-sT cells. Similarly, HCAEC wounds closed to 29, 49, or 33% (S-W-sH) compared with 58% in the S-W-sT cells. Cell spreading and migration, but not proliferation, were the major mechanisms accounting for the increases in wound closure rate. These results suggest that physiological levels of shear stress enhance endothelial repair.This publication has 27 references indexed in Scilit:
- Shear stress and the endotheliumKidney International, 1998
- Fibrin(ogen) and von Willebrand Factor Deposition Are Associated With Intimal Thickening After Balloon Angioplasty of the Rabbit Carotid ArteryArteriosclerosis, Thrombosis, and Vascular Biology, 1997
- Flow-mediated endothelial mechanotransductionPhysiological Reviews, 1995
- Adhesion and Differentiation of Endothelial Cells by Exposure to Chronic Shear Stress: A Vascular Graft ModelBlood Purification, 1995
- Endothelial Wounds with Disruption in Cell Migration Repair Primarily by Cell ProliferationMicrovascular Research, 1994
- Hemodynamics and AtherogenesisAnnals of the New York Academy of Sciences, 1994
- The Role of Fluid Mechanics in the Localization and Detection of AtherosclerosisJournal of Biomechanical Engineering, 1993
- Shear stress induced stimulation of mammalian cell metabolismBiotechnology & Bioengineering, 1988
- Influence of hemodynamic forces on vascular endothelial function. In vitro studies of shear stress and pinocytosis in bovine aortic cells.JCI Insight, 1984
- Arterial Wall Shear and Distribution of Early Atheroma in ManNature, 1969