Lessened malate inhibition of guard‐cell phosphoenolpyruvate carboxylase velocity during stomatal opening
- 19 September 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 352 (1), 45-48
- https://doi.org/10.1016/0014-5793(94)00916-3
Abstract
Leaflets of Vicia faba with closed stomata or with opening stomata were freeze-dried. Excised guard-cell pairs were assayed individually under suboptimal conditions (pH 7.1 and subsaturating substrate) for phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) using quantitative histochemical procedures. l-Malate, 400 μM, significantly inhibited guard-cell PEPC activity of closed stomata but not that of opening stomata. We postulate that the lessened sensitivity of guard-cell PEPC activity to malate inhibition is an important regulatory feature of stomatal opening, which is associated with malate accumulation.Keywords
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