3D scanning probe nanotomography of tissue spheroid fibroblasts interacting with electrospun polyurethane scaffold
- 1 January 2019
- journal article
- research article
- Published by Department of Polymer Engineering, Scientific Society of Mechanical Engineering in Express Polymer Letters
- Vol. 13 (7), 632-641
- https://doi.org/10.3144/expresspolymlett.2019.53
Abstract
We present a 3D study of nanostructural features of a bioprinted tissue spheroid interacting with polyurethane dual-scale biocompatible scaffold manufactured by three-dimensional printing and electrospinning. Three-dimensional analysis of fibroblasts interacting with electrospun polyurethane fibers was conducted using scanning probe nanotomography with an experimental setup combining ultramicrotome and a scanning probe microscope. Three-dimensional reconstruction demonstrates direct visualization of cell membrane protrusions and coherent cell-fiber interfaces, the formation of which is a prerequisite for an efficient tissue engineered implant. Analysis of obtained 3D data allows for quantitative calculation of the important morphological parameters of adhered cells, scaffolds, and cell-scaffold interfaces. The proposed method may be successfully applied to investigate 3D cell-scaffold constructs at nanoscale.Keywords
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