Soluble N‐cadherin in human biological fluids
Open Access
- 26 October 2006
- journal article
- early detection-and-diagnosis
- Published by Wiley in International Journal of Cancer
- Vol. 119 (12), 2895-2900
- https://doi.org/10.1002/ijc.22219
Abstract
Classical cadherins such as E‐, P‐ and N‐cadherin are transmembrane proteins that mediate cell–cell adhesion, and are important in embryogenesis, maintenance of tissue integrity and cancer. Proteolytic shedding of the extracellular domain results in the generation of soluble E‐, P‐ or N‐cadherin ectodomains. Circulating soluble E‐ and P‐cadherin have been described in the serum, and elevated levels were detected in cancer patients when compared with healthy persons. Here we report the presence of soluble N‐cadherin, a 90‐kD protein fragment, in the serum of both healthy persons and cancer patients, using a direct ELISA and immunoprecipitation. A correlation was found between prostate specific antigen and soluble N‐cadherin, and significantly elevated levels were detected in prostate cancer follow‐up patients. The N‐cadherin protein is neo‐expressed by carcinomas of the prostate, and is responsible for epithelial to fibroblastic transition. This is reflected by the higher concentrations of soluble N‐cadherin in prostate cancer patients than in healthy persons.Keywords
This publication has 29 references indexed in Scilit:
- Membrane Localization of Membrane Type 5 Matrix Metalloproteinase by AMPA Receptor Binding Protein and Cleavage of CadherinsJournal of Neuroscience, 2006
- Ischemia-induced cleavage of cadherins in NRK cells requires MT1-MMP (MMP-14)American Journal of Physiology-Renal Physiology, 2006
- N-cadherin switching occurs in high Gleason grade prostate cancerThe Prostate, 2005
- ADAM10 mediates E-cadherin shedding and regulates epithelial cell-cell adhesion, migration, and β-catenin translocationProceedings of the National Academy of Sciences of the United States of America, 2005
- ADAM10 cleavage of N-cadherin and regulation of cell–cell adhesion and β-catenin nuclear signallingThe EMBO Journal, 2005
- N-cadherin in the spotlight of cell-cell adhesion, differentiation, embryogenesis, invasion and signallingThe International Journal of Developmental Biology, 2004
- Plasmin Produces an E-Cadherin Fragment That Stimulates Cancer Cell InvasionBiological Chemistry, 2002
- Soluble E-cadherin concentrations in patients with systemic inflammatory response syndrome and multiorgan dysfunction syndromeBritish Journal of Anaesthesia, 1996
- Soluble E-cadherin fragments increased in circulation of cancer patientsBritish Journal of Cancer, 1994
- Purification and characterization of NCAD90, a Soluble endogenous form of N‐cadherin, which is generated by proteolysis during retinal development and retains adhesive and neurite‐promoting functionJournal of Neuroscience Research, 1993