Activation of the guinea pig alternative complement pathway by mouse IgA immune complexes.
Open Access
- 1 January 1982
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 155 (1), 231-247
- https://doi.org/10.1084/jem.155.1.231
Abstract
Activation of the complement system by IgA was investigated with immune complexes containing a mouse IgA myeloma protein with specificity for phosphorylcholine linked to bovine serum albumin (PC-BSA). These IgA anti-PC-BSA immune complexes activated the alternative complement pathway in mouse and guinea pig serum, while human complement was not affected. The activation proceeded with consumption of C3 but little or no consumption of C5. C3 did not bind to the IgA immune complexes during complement activation although it did bind covalently to IgG immune complexes. It is suggested that IgA immune complexes do not supply a suitable surface for C3 binding and effective alternative pathway convertase assembly; therefore, cleavage is limited and occurs primarily in the fluid phase. Without C3 binding, C5 cleavage does not occur nor can the alternative pathway activation proceed to the amplification step.Keywords
This publication has 48 references indexed in Scilit:
- Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-SepharoseImmunochemistry, 1978
- Interaction between the third complement protein and cell surface macromolecules.Proceedings of the National Academy of Sciences of the United States of America, 1977
- Mechanism of Solubilization of Immune Aggregates by Complement. Implications for ImmunopathologyImmunological Reviews, 1976
- Surface and intracellular markers of mouse mesenteric and peripheral lymph node and Peyer's patch cells.1974
- The Complement System in Necrotizing Angiitis of the Skin. Analysis of Complement Component Activities in Serum of Patients with Concomitant Collagen-Vascular DiseasesJournal of Investigative Dermatology, 1974
- Protein iodination with solid state lactoperoxidaseBiochemistry, 1974
- THE RELATIVE ANTIBACTERIAL EFFICIENCY OF IgM, IgG AND IgA FROM PIG COLOSTRUMAustralian Journal of Experimental Biology and Medical Science, 1971
- THE REACTION MECHANISM OF HUMAN C5 IN IMMUNE HEMOLYSISThe Journal of Experimental Medicine, 1970
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Hemolytic Activity of Lipoprotein-Depleted Serum and the Effect of Certain Anions on ComplementThe Journal of Immunology, 1966