Resistance of Red Clover (Trifolium pratense) to the Root Parasitic Plant Orobanche minor is Activated by Salicylate but not by Jasmonate
Open Access
- 27 July 2007
- journal article
- research article
- Published by Oxford University Press (OUP) in Annals of Botany
- Vol. 100 (3), 537-544
- https://doi.org/10.1093/aob/mcm148
Abstract
Obligate root holoparasites of the genus Orobanche attack dicotyledonous crops and cause severe losses in many parts of the world. Chemical induction of plant defence systems such as systemic acquired resistance was proposed to be an available strategy to control the root parasite, but the detailed mechanisms involved have not been clarified. The aim of this study was to elucidate the effects of salicylic acid (SA), jasmonic acid (JA) and their analogues on resistance of red clover to Orobanche parasitism. Roots of red clover grown in plastic chambers were applied with SA, S-methyl benzo[1,2,3]thiadiazole-7-carbothioate (BTH), methyl jasmonate (MeJA) and n-propyl dihydrojasmonate (PDJ), and then were inoculated with O. minor seeds. Attachments of the parasite were observed after 5 weeks. SA and BTH, inducers of SA-mediated defences, significantly reduced the number of established parasites by more than 75 %. By contrast, MeJA and PDJ, inducers of JA-mediated defences, did not affect parasitism. The reduction in the number of established parasites by SA and BTH was due to the inhibited elongation of O. minor radicles and the activation of defence responses in the host root including lignification of the endodermis. These results suggest that SA-induced resistance, but not JA-induced resistance, is effective in inhibiting Orobanche parasitism and that the resistance is expressed by the host root both externally and internally.Keywords
This publication has 15 references indexed in Scilit:
- Pre-haustorial resistance to broomrape (Orobanche cumana) in sunflower (Helianthus annuus): cytochemical studiesJournal of Experimental Botany, 2006
- Protein cross-linking, peroxidase and β-1,3-endoglucanase involved in resistance of pea against Orobanche crenataJournal of Experimental Botany, 2006
- Mucilage production during the incompatible interaction between Orobanche crenata and Vicia sativaJournal of Experimental Botany, 2006
- An Improved Axenic System for Studying Pre-infection Development of the Parasitic Plant Orobanche ramosaAnnals of Botany, 2005
- Defense Gene Expression Analysis of Arabidopsis thaliana Parasitized by Orobanche ramosaPhytopathology®, 2003
- Separate jasmonate-dependent and salicylate-dependent defense-response pathways in Arabidopsis are essential for resistance to distinct microbial pathogensProceedings of the National Academy of Sciences of the United States of America, 1998
- Jasmonate and salicylate as global signals for defense gene expressionCurrent Opinion in Plant Biology, 1998
- Jasmonate is essential for insect defense in ArabidopsisProceedings of the National Academy of Sciences of the United States of America, 1997
- Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat.THE PLANT CELL ONLINE, 1996
- Production of Salicylic Acid Precursors Is a Major Function of Phenylalanine Ammonia-Lyase in the Resistance of Arabidopsis to Peronospora parasitica.Plant Cell, 1996