Crystal structures of HIV protease V82A and L90M mutants reveal changes in the indinavir‐binding site

Abstract

The crystal structures of the wild‐type HIV‐1 protease (PR) and the two resistant variants, PR_V82A and PR_L90M, have been determined in complex with the antiviral drug, indinavir, to gain insight into the molecular basis of drug resistance. V82A and L90M correspond to an active site mutation and nonactive site mutation, respectively. The inhibition (K_i) of PR_V82A and PR_L90M was 3.3‐ and 0.16‐fold, respectively, relative to the value for PR. They showed only a modest decrease, of 10–15%, in their k_cat/K_m values relative to PR. The crystal structures were refined to resolutions of 1.25–1.4 Å to reveal critical features associated with inhibitor resistance. PR_V82A showed local changes in residues 81–82 at the site of the mutation, while PR_L90M showed local changes near Met90 and an additional interaction with indinavir. These structural differences concur with the kinetic data.