Prevalence of Shigella Enterotoxins 1 and 2 among Shigella Strains Isolated from Patients with Traveler's Diarrhea
Open Access
- 1 November 1999
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 37 (11), 3608-3611
- https://doi.org/10.1128/jcm.37.11.3608-3611.1999
Abstract
Shigella spp. are known primarily as a cause of bacillary dysentery. However, in an initial phase, numerous patients exhibit watery diarrhea that may or may not be followed by dysentery. New virulence factors associated with the species of Shigella have recently been described. These are enterotoxins 1 and 2 of Shigella (ShET-1 and ShET-2, respectively). The aim of the present study was to determine the prevalence of ShET-1 and ShET-2 in species of Shigella isolated from patients with traveler's diarrhea. During the period from 1993 to 1998, stool samples from 500 travelers with diarrhea were cultured for the isolation of Shigella spp. and other enteropathogens. The detection of ShET-1 and ShET-2 was performed by a PCR technique with specific primers. Among a total of 51 strains of Shigella isolated during this period (22 S. flexneri , 26 S. sonnei , and 3 S. dysenteriae strains), at least one enterotoxin was detected in 31 (60.78%) strains; 2 (9.09%; both of which were S. flexneri strains) produced only ShET-1, while 21 (41.17%; 3 S. flexneri , 15 S. sonnei , and 3 S. dysenteriae strains) produced ShET-2. Furthermore, 8 (15.69%) of 22 S. flexneri strains presented both enterotoxins. Our results show that the prevalence of ShET-2 was high in all the Shigella species studied and confirm that ShET-1 is detected only in S. flexneri.Keywords
This publication has 12 references indexed in Scilit:
- Manual of Clinical Microbiology, 8th Edition:Manual of Clinical Microbiology, 8th EditionClinical Infectious Diseases, 2004
- Prevalence of Shigella Enterotoxin 1 among Shigella Clinical Isolates of Diverse SerotypesThe Journal of Infectious Diseases, 1995
- Shigella enterotoxin 1: an enterotoxin of Shigella flexneri 2a active in rabbit small intestine in vivo and in vitro.JCI Insight, 1995
- Antimicrobial resistance of Shigella isolates causing traveler's diarrheaAntimicrobial Agents and Chemotherapy, 1994
- Detection of shigellae, enteroinvasive and enterotoxigenic Escherichia coli using the polymerase chain reaction (PCR) in patients returning from tropical countriesMolecular and Cellular Probes, 1994
- Etiology of traveller's diarrhea in Spanish travellers to developing countriesEuropean Journal of Epidemiology, 1993
- Antimicrobial Resistance of Shigella Isolates in the USA: The Importance of International TravelersThe Journal of Infectious Diseases, 1990
- Multi‐gene amplification: simultaneous detection of three virulence genes in diarrhoeal stoolMolecular Microbiology, 1989
- Shiga Toxin -- An Expanding Role in the Pathogenesis of Infectious DiseasesThe Journal of Infectious Diseases, 1985
- ShigellosisPublished by Elsevier BV ,1984