Structure-based engineering of papillomavirus major capsid L1: controlling particle assembly
Open Access
- 8 January 2007
- journal article
- Published by Springer Science and Business Media LLC in Virology Journal
- Vol. 4 (1), 3
- https://doi.org/10.1186/1743-422x-4-3
Abstract
The outer shell of the papillomavirus particle is comprised of 72 pentamers of the major capsid L1 protein arranged on a T = 7 icosahedral lattice. The recombinant L1 can form T = 7 virus-like particles in vitro. The crystal structure of a T = 7 papilloma virion has not yet been determined; however, the crystal structure of a T = 1 particle containing 12 pentamers is known. The T = 1 structure reveals that helix-helix interactions, through three helices–h2, h3, and h4–near the C-terminus of L1, mediate the inter-pentameric bonding that is responsible for T = 1 assembly. Based on the T = 1 crystal structure, we have generated a set of internal deletions to test the role of the three C-terminal helices in T = 7 assembly. We have demonstrated that the h2, h3, and h4 near the C-terminal end of L1 are important for the L1 structure and particle assembly. In particular, we found that h2 and h3 are essential for L1 folding and pentamer formation, whereas h4 is indispensable for the assembly of not only T1, but also of the T7 virus-like particle.Keywords
This publication has 16 references indexed in Scilit:
- Atomic model of the papillomavirus capsidThe EMBO Journal, 2002
- Papillomavirus capsid protein expression in Escherichia coli: purification and assembly of HPV11 and HPV16 L1Journal of Molecular Biology, 2001
- Assembly of Human Papillomavirus Type 16 Pseudovirions inSaccharomyces cerevisiaeHuman Gene Therapy, 2000
- Structure of Small Virus-like Particles Assembled from the L1 Protein of Human Papillomavirus 16Molecular Cell, 2000
- Infectious human papillomavirus type 18 pseudovirionsJournal of Molecular Biology, 1998
- Novel structural features of bovine papillomavirus capsid revealed by a three-dimensional reconstruction to 9 Å resolutionNature Structural & Molecular Biology, 1997
- Structure of murine polyomavirus complexed with an oligosaccharide receptor fragmentNature, 1994
- Synthesis and assembly of infectious bovine papillomavirus particles in vitroJournal of General Virology, 1993
- Papillomavirus L1 major capsid protein self-assembles into virus-like particles that are highly immunogenic.Proceedings of the National Academy of Sciences of the United States of America, 1992
- Structure of simian virus 40 at 3.8-Å resolutionNature, 1991