Oxidant/Antioxidant Properties of Croatian Native Propolis
- 27 September 2006
- journal article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 54 (21), 8018-8026
- https://doi.org/10.1021/jf0612023
Abstract
Native propolis was defined as propolis powder collected from the continental part of Croatia and prepared according to a patented process that preserves all the propolis natural nutritional and organoleptic qualities. Nine phenolic compounds (out of thirteen tested) in propolis sample were detected by high performance liquid chromatography (HPLC) analysis. Among them chrysin was the most abundant (2478.5 μg/g propolis). Contrary to moderate antioxidant activity of propolis examined in vitro (ferric reduction antioxidant power; FRAP-assay), propolis as a food supplement modulated antioxidant enzymes (AOE) and significantly decreased lipid peroxidation processes (LPO) in plasma, liver, lungs, and brain of mice. The effect was dose- and tissue-dependent. The lower dose (100 mg/kg bw) protected plasma from oxidation, whereas the higher dose (300 mg/kg bw) was pro-oxidative. Hyperoxia (long-term normobaric 100% oxygen) increased LPO in all three organs tested. The highest vulnerability to oxidative stress was observed in lungs where hyperoxia was not associated with augmentation of AOE. Propolis protected lungs from hyperoxia by increased catalase (CAT) activity. This is of special importance for lungs since lungs of adult animals are highly vulnerable to oxidative stress because of their inability to augment AOE activity. Because of its strong antioxidant and scavenging abilities, native propolis might be used as a strong plant-based antioxidant effective not only in physiological conditions but also in cases that require prolonged high concentration of oxygen. Keywords: Propolis; antioxidant enzymes; lipid peroxidation; flavonoids.Keywords
This publication has 26 references indexed in Scilit:
- Flavonoids Are Inhibitors of Breast Cancer Resistance Protein (ABCG2)-Mediated TransportMolecular Pharmacology, 2004
- The effects of caffeic acid phenethyl ester on tissue damage in lung after hindlimb ischemia-reperfusionPharmacological Research, 2003
- Curcumin activates the haem oxygenase-1 gene via regulation of Nrf2 and the antioxidant-responsive elementBiochemical Journal, 2003
- Antibacterial activity of Brazilian propolis and fractions against oral anaerobic bacteriaJournal of Ethnopharmacology, 2002
- Flavonoids can replace α‐tocopherol as an antioxidantFEBS Letters, 2000
- Anti- and pro-oxidative effects of flavonoids on metal-induced lipid hydroperoxide-dependent lipid peroxidation in cultured hepatocytes loaded with α-linolenic acidFree Radical Biology & Medicine, 1999
- Anxiolytic Natural and Synthetic Flavonoid Ligands of the Central Benzodiazepine Receptor Have No Effect on Memory Tasks in RatsPharmacology Biochemistry and Behavior, 1997
- SERUM BIOCHEMICAL DETERMINATIONS OF PROPOLIS-TREATED RATSJournal of Venomous Animals and Toxins including Tropical Diseases, 1995
- Scavenging action of propolis extract against oxygen radicalsJournal of Ethnopharmacology, 1994
- Normobaric Oxygen Toxicity of the LungNew England Journal of Medicine, 1980