Synergism betweenClostridiwn Thermocellum cellulases cloned inEscherichia coli
- 1 November 1992
- journal article
- Published by Springer Science and Business Media LLC in Applied Biochemistry and Biotechnology
- Vol. 37 (2), 201-207
- https://doi.org/10.1007/bf02921671
Abstract
We have obtained a synergistic effect during degradation of Avicel and filter paper byClostridium thermocellum cellulases (two endoglu-canases and one cellobiohydrolase) cloned inEscherichia coli. The highest degree of synergism was found at early stages of reaction, during the first 20 h: 2.5 and 2.9 on Avicel and filter paper, respectively. During combined action of all three cellulases the main product is cellobiose.Keywords
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