Osteopontin Localization in the Holstein Bull Reproductive Tract1
- 1 February 1999
- journal article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 60 (2), 454-460
- https://doi.org/10.1095/biolreprod60.2.454
Abstract
Previously we reported that the 55-kDa fertility-associated protein in Holstein bull seminal plasma (SP) is osteopontin (OPN). The objective of the present study was to localize OPN in tissues and fluids in the Holstein bull reproductive tract to determine its origin. Antisera generated against human recombinant OPN, as well as antiserum prepared against purified bovine seminal plasma OPN, reacted with protein in SP, accessory sex gland fluid, seminal vesicle fluid, ampullary fluid, and urine using one- and two-dimensional SDS-PAGE Western blot analysis. However, these antisera failed to detect OPN in cauda epididymal fluid or solubilized sperm membranes. Immunofluorescence histochemistry localized OPN in the lumen and epithelial cells of the seminal vesicle and ampulla, but not in tissues of testis, epididymis, prostate, and bulbourethral gland. OPN was not detected immunohistochemically in epididymal, ampullary, or ejaculated sperm treated with or without Triton X-100. We concluded that the primary sources of OPN in bull SP are the seminal vesicles and ampulla.Keywords
This publication has 17 references indexed in Scilit:
- Osteopontin is the 55-Kilodalton Fertility-Associated Protein in Holstein Bull Seminal Plasma1Biology of Reproduction, 1997
- Fertility-Associated Proteins in Holstein Bull Seminal Plasma1Biology of Reproduction, 1993
- Expression and distribution of osteopontin in human tissues: widespread association with luminal epithelial surfaces.Molecular Biology of the Cell, 1992
- Molecular cloning and sequencing of cDNA encoding urinary stone protein, which is identical to osteopontinBiochemical and Biophysical Research Communications, 1992
- Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily.Proceedings of the National Academy of Sciences of the United States of America, 1992
- The cDNA cloning and RNA distribution of bovine osteopontinGene, 1991
- The murine gene encoding secreted phosphoprotein 1 (osteopontin): promoter structure, activity, and induction in vivo by estrogen and progesteroneGene, 1991
- cDNA cloning, mRNA distribution and heterogeneity, chromosomal location, and RFLP analysis of human osteopontin (OPN)Genomics, 1990
- Developmental expression of 2ar (osteopontin) and SPARC (osteonectin) RNA as revealed by in situ hybridizationThe Journal of cell biology, 1988
- Tissue specificity and developmental expression of rat osteopontinBiochemical and Biophysical Research Communications, 1987