Methods for the Study of Dopamine Receptors Within Lipid Rafts of Kidney Cells
Open Access
- 30 October 2012
- book chapter
- research article
- Published by Springer Science and Business Media LLC
- Vol. 964, 15-24
- https://doi.org/10.1007/978-1-62703-251-3_2
Abstract
There is increasing evidence that G protein-coupled receptor (GPCR) signaling is regulated in lipid raft microdomains. GPCRs and GPCR-signaling molecules, including G proteins and protein kinases, have been reported to compartmentalize in these microdomains. Dopamine D1-like receptors (D1R and D5R) belong to a family of GPCRs that are important in the regulation of renal function. These receptors are not only localized and regulated in caveolae that contains caveolin-1 but are also distributed in non-caveolar lipid rafts which do not contain caveolin-1. This chapter describes detergent- and non-detergent-based methods to obtain lipid raft fractions from renal proximal tubule cells.Keywords
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