Identification of the lesion in the stimulatory GTP‐binding protein of the uncoupled S49 lymphoma
- 30 November 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 224 (2), 365-371
- https://doi.org/10.1016/0014-5793(87)80486-6
Abstract
The stimulatory GTP-binding protein (Gs) of the uncoupled mutant of S49 lymphoma cells is deficient in its ability to transduce hormonal signals from ligand-bound β-adrenergic receptors to the catalytic component of adenylate cyclase. In order to define the genetic defect in the Gs of uncoupled S49 cells, a complementary DNA clone encoding the α-subunit of Gs was analyzed and the deduced primary structure of the defective subunit compared to that of the wild-type subunit. A single nucleotide transversion was found that coded for a proline rather than an arginine at residue 389. The results indicate a domain of the α-subunit of Gs that specifically interacts with hormone receptorsKeywords
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