Potential Cell Culture Models for Antioxidant Research
- 26 April 2005
- journal article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 53 (10), 4311-4314
- https://doi.org/10.1021/jf058070i
Abstract
The antioxidant activity of pure compounds, foods, and dietary supplements has been extensively studied with the development of many new antioxidant and antioxidant activity assays in recent years. However, these assays, such as total phenolics, total flavonoids, and total antioxidant activity in vitro, do not reflect the cellular physiological conditions and do not consider the bioavailability and metabolism issues. In addition, the mechanisms of action of antioxidants go beyond the antioxidant activity scavenging free radicals in disease prevention and health promotion. Animal models and human studies are expensive and not suitable for initial antioxidant screening of foods and dietary supplements. Therefore, there is a need for cell culture models to access the bioactivity of antioxidants. This paper is an overview of cell culture models for antioxidant research, as reported at the First International Congress on Antioxidant Methods, held in Orlando, FL, June 16−18, 2004, and outlines potential cell culture models for initial antioxidant screening and antioxidant research. Keywords: Antioxidants; foods; antioxidant activity; cancer; cardiovascular disease; cell culture modelsThis publication has 12 references indexed in Scilit:
- In vitro digestion and lactase treatment influence uptake of quercetin and quercetin glucoside by the Caco-2 cell monolayerNutrition Journal, 2005
- Uptake of Quercetin and Quercetin 3-Glucoside from Whole Onion and Apple Peel Extracts by Caco-2 Cell MonolayersJournal of Agricultural and Food Chemistry, 2004
- Assays for Hydrophilic and Lipophilic Antioxidant Capacity (oxygen radical absorbance capacity (ORACFL)) of Plasma and Other Biological and Food SamplesJournal of Agricultural and Food Chemistry, 2003
- Comparison of C-Reactive Protein and Low-Density Lipoprotein Cholesterol Levels in the Prediction of First Cardiovascular EventsThe New England Journal of Medicine, 2002
- Balancing Life-Style and Genomics Research for Disease PreventionScience, 2002
- Transport of Proanthocyanidin Dimer, Trimer, and Polymer Across Monolayers of Human Intestinal Epithelial Caco-2 CellsAntioxidants and Redox Signaling, 2001
- Study of low-density lipoprotein oxidizability indexes to measure the antioxidant activity of dietary polyphenolsNutrition Research, 2000
- Antioxidants and disease: More questions than answersNutrition Research, 2000
- Accumulation and retention of micellar β-carotene and lutein by Caco-2 human intestinal cellsThe Journal of Nutritional Biochemistry, 1999
- Fruit, vegetables, and cancer prevention: A review of the epidemiological evidenceNutrition and Cancer, 1992