Portable and Quantitative Detection of Protein Biomarkers and Small Molecular Toxins Using Antibodies and Ubiquitous Personal Glucose Meters
- 20 March 2012
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 84 (9), 4174-4178
- https://doi.org/10.1021/ac300517n
Abstract
Developing portable and low-cost methods for quantitative detection of large protein biomarkers and small molecular toxins can play a significant role in controlling and preventing diseases or toxins outbreaks. Despite years of research, most current methods still require laboratory-based or customized devices that are not widely available to the general public for quantitative analysis. We have previously demonstrated the use of personal glucose meters (PGMs) and functional DNAs for the detection of many nonglucose targets. However, the range of targets detectable by functional DNAs is limited at the current stage. To expand the range of targets that can be detected by PGMs, we report here the use of antibodies in combination with sandwich and competitive assays for quantitative detection of protein biomarkers (PSA, with a detection limit of 0.4 ng/mL) and small molecular toxins (Ochratoxin A, with a detection limit of 6.8 ng/mL), respectively. In both assay methods, with invertase conjugates as the link, quantitative detection is achieved via the dependence between the concentrations of the targets in the sample and the glucose measured by PGMs. Given the wide availability of antibodies for numerous targets, the methods demonstrated here can expand the range of target detection by PGMs significantly.This publication has 34 references indexed in Scilit:
- Using Commercially Available Personal Glucose Meters for Portable Quantification of DNAAnalytical Chemistry, 2012
- Using personal glucose meters and functional DNA sensors to quantify a variety of analytical targetsNature Chemistry, 2011
- Integration of paper-based microfluidic devices with commercial electrochemical readersLab on a Chip, 2010
- Nanoparticle-based bio-barcode assay redefines “undetectable” PSA and biochemical recurrence after radical prostatectomyProceedings of the National Academy of Sciences, 2009
- Prostate Specific Antigen Detection Using Microgapped Electrode Array Immunosensor with Enzymatic Silver DepositionClinical Chemistry, 2009
- Functional Nucleic Acid SensorsChemical Reviews, 2009
- Chemiluminescence immunoassayTrAC Trends in Analytical Chemistry, 2009
- Carbon Nanotube Amplification Strategies for Highly Sensitive Immunodetection of Cancer BiomarkersJournal of the American Chemical Society, 2006
- Development of a bienzyme reactor sensor system for the determination of ornithineAnalytica Chimica Acta, 2002
- Ochratoxin A: A review (Technical Report)Published by Walter de Gruyter GmbH ,1992