Clostridium acidurici Electron-Bifurcating Formate Dehydrogenase
Open Access
- 1 October 2013
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 79 (19), 6176-6179
- https://doi.org/10.1128/aem.02015-13
Abstract
Cell extracts of uric acid-grown Clostridium acidurici catalyzed the coupled reduction of NAD + and ferredoxin with formate at a specific activity of 1.3 U/mg. The enzyme complex catalyzing the electron-bifurcating reaction was purified 130-fold and found to be composed of four subunits encoded by the gene cluster hylCBA-fdhF2 .This publication has 34 references indexed in Scilit:
- The Purine-Utilizing Bacterium Clostridium acidurici 9a: A Genome-Guided Metabolic ReconsiderationPLOS ONE, 2012
- New Insights into [FeFe] Hydrogenase Activation and Maturase FunctionPLOS ONE, 2012
- A Bacterial Electron-bifurcating HydrogenaseJournal of Biological Chemistry, 2012
- Electron Bifurcation Involved in the Energy Metabolism of the Acetogenic Bacterium Moorella thermoacetica Growing on Glucose or H 2 plus CO 2Journal of Bacteriology, 2012
- Coupling of ferredoxin and heterodisulfide reduction via electron bifurcation in hydrogenotrophic methanogenic archaeaProceedings of the National Academy of Sciences of the United States of America, 2011
- NADP+Reduction with Reduced Ferredoxin and NADP+Reduction with NADH Are Coupled via an Electron-Bifurcating Enzyme Complex inClostridium kluyveriJournal of Bacteriology, 2010
- Protein complexing in a methanogen suggests electron bifurcation and electron delivery from formate to heterodisulfide reductaseProceedings of the National Academy of Sciences of the United States of America, 2010
- The Iron-Hydrogenase of Thermotoga maritima Utilizes Ferredoxin and NADH Synergistically: a New Perspective on Anaerobic Hydrogen ProductionJournal of Bacteriology, 2009
- Energy Conservation via Electron-Transferring Flavoprotein in Anaerobic BacteriaJournal of Bacteriology, 2008
- Coupled Ferredoxin and Crotonyl Coenzyme A (CoA) Reduction with NADH Catalyzed by the Butyryl-CoA Dehydrogenase/Etf Complex from Clostridium kluyveriJournal of Bacteriology, 2008