Recombinant human bone morphogenetic protein induces bone formation.
- 1 March 1990
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 87 (6), 2220-2224
- https://doi.org/10.1073/pnas.87.6.2220
Abstract
We have purified and characterized active recombinant human bone morphogenetic protein (BMP) 2A. Implantation of the recombinant protein in rats showed that a single BMP can induce bone formation in vivo. A dose-response and time-course study using the rat ectopic bone formation assay revealed that implantation of 0.5-115 micrograms of partially purified recombinant human BMP-2A resulted in cartilage by day 7 and bone formation by day 14. The time at which bone formation occurred was dependent on the amount of BMP-2A implanted; at high doses bone formation could be observed at 5 days. The cartilage- and bone-inductive activity of the recombinant BMP-2A is histologically indistinguishable from that of bone extracts. Thus, recombinant BMP-2A has therapeutic potential to promote de novo bone formation in humans.Keywords
This publication has 20 references indexed in Scilit:
- Novel Regulators of Bone Formation: Molecular Clones and ActivitiesScience, 1988
- Synthetic peptides as antigens: Pitfalls of conjugation methodsJournal of Immunological Methods, 1985
- Purification of bovine bone morphogenetic protein by hydroxyapatite chromatography.Proceedings of the National Academy of Sciences of the United States of America, 1984
- Homology of bone-inductive proteins from human, monkey, bovine, and rat extracellular matrix.Proceedings of the National Academy of Sciences of the United States of America, 1983
- Bone Cell Differentiation and Growth FactorsScience, 1983
- A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gelsAnalytical Biochemistry, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences of the United States of America, 1979
- Solubilized and insolubilized bone morphogenetic protein.Proceedings of the National Academy of Sciences of the United States of America, 1979
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970