An Updated Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of H5N1 Avian Influenza Viruses
Open Access
- 1 January 2011
- journal article
- Published by Japanese Society of Tropical Medicine in Tropical Medicine and Health
- Vol. 39 (1), 3-7
- https://doi.org/10.2149/tmh.2010-21
Abstract
We designed a new set of primers for reverse transcriptase loop-mediated isothermal amplification (RTLAMP) to specifically amplify the HA gene of avian influenza viruses subtype H5N1. By testing nine H5N1 virus strains and 41 clinical samples collected in Northern Vietnam, we found that the new primers showed higher sensitivity and specificity than the previously published RT-LAMP primers and were comparable to the RT-PCR method currently recommended by WHO. These results suggest that our RT-LAMP assay may be a better choice as a diagnostic tool for current H5N1 influenza virus infection.Keywords
This publication has 11 references indexed in Scilit:
- Antigenic and genetic characteristics of influenza A(H5N1) and influenza A(H9N2) viruses and candidate vaccine viruses developed for potential use in human vaccines - February 2010.2010
- Development and Evaluation of a Real-Time Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Rift Valley Fever Virus in Clinical SpecimensJournal of Clinical Microbiology, 2009
- Loop-Mediated Isothermal Amplification for Influenza A (H5N1) VirusEmerging Infectious Diseases, 2007
- Rapid diagnosis of H5N1 avian influenza virus infection by newly developed influenza H5 hemagglutinin gene-specific loop-mediated isothermal amplification methodJournal of Virological Methods, 2007
- Antigenic and genetic characteristics of H5N1 viruses and candidate H5N1 vaccine viruses developed for potential use as pre-pandemic vaccines, March 2007.2007
- Development of H5-RT-LAMP (loop-mediated isothermal amplification) system for rapid diagnosis of H5 avian influenza virus infectionVaccine, 2006
- Real-time turbidimetry of LAMP reaction for quantifying template DNAJournal of Biochemical and Biophysical Methods, 2004
- Development and Evaluation of a Novel Loop-Mediated Isothermal Amplification Method for Rapid Detection of Severe Acute Respiratory Syndrome CoronavirusJournal of Clinical Microbiology, 2004
- Real-Time Reverse Transcription Loop-Mediated Isothermal Amplification for Rapid Detection of West Nile VirusJournal of Clinical Microbiology, 2004
- Loop-mediated isothermal amplification of DNANucleic Acids Research, 2000