Are luminescent bacteria suitable for online detection and monitoring of toxic compounds in drinking water and its sources?
Open Access
- 9 November 2010
- journal article
- review article
- Published by Springer Science and Business Media LLC in Analytical and Bioanalytical Chemistry
- Vol. 400 (4), 915-929
- https://doi.org/10.1007/s00216-010-4372-6
Abstract
Biosensors based on luminescent bacteria may be valuable tools to monitor the chemical quality and safety of surface and drinking water. In this review, an overview is presented of the recombinant strains available that harbour the bacterial luciferase genes luxCDABE, and which may be used in an online biosensor for water quality monitoring. Many bacterial strains have been described for the detection of a broad range of toxicity parameters, including DNA damage, protein damage, membrane damage, oxidative stress, organic pollutants, and heavy metals. Most lux strains have sensitivities with detection limits ranging from milligrams per litre to micrograms per litre, usually with higher sensitivities in compound-specific strains. Although the sensitivity of lux strains can be enhanced by various molecular manipulations, most reported detection thresholds are still too high to detect levels of individual contaminants as they occur nowadays in European drinking waters. However, lux strains sensing specific toxic effects have the advantage of being able to respond to mixtures of contaminants inducing the same effect, and thus could be used as a sensor for the sum effect, including the effect of compounds that are as yet not identified by chemical analysis. An evaluation of the suitability of lux strains for monitoring surface and drinking water is therefore provided.Keywords
This publication has 76 references indexed in Scilit:
- Bacterial genotoxicity bioreportersMicrobial Biotechnology, 2010
- Where microbiology meets microengineering: design and applications of reporter bacteriaNature Reviews Microbiology, 2010
- A suite of recombinant luminescent bacterial strains for the quantification of bioavailable heavy metals and toxicity testingBMC Biotechnology, 2009
- A novel bioluminescent bacterial biosensor using the highly specific oxidative stress-inducible pgi geneBiosensors and Bioelectronics, 2008
- EU-wide survey of polar organic persistent pollutants in European river watersEnvironmental Pollution, 2008
- An oxidative stress-specific bacterial cell array chip for toxicity analysisBiosensors and Bioelectronics, 2007
- Fabrication of a bio-MEMS based cell-chip for toxicity monitoringBiosensors and Bioelectronics, 2007
- A Multi-Channel Continuous Water Toxicity Monitoring System: Its Evaluation and Application to Water Discharged from a Power PlantEnvironmental Monitoring and Assessment, 2005
- Detection of bioavailable heavy metals in EILATox‐Oregon samples using whole‐cell luminescent bacterial sensors in suspension or immobilized onto fibre‐optic tipsJournal of Applied Toxicology, 2004
- Illuminating the detection chain of bacterial bioreportersEnvironmental Microbiology, 2004