Α molecular epidemiological analysis of adenoviruses from excess conjunctivitis cases
Open Access
- 24 April 2017
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Ophthalmology
- Vol. 17 (1), 51
- https://doi.org/10.1186/s12886-017-0447-x
Abstract
Τo perform a molecular epidemiological analysis of viral conjunctivitis among excess conjunctivitis cases recorded at the University Hospital of Patras, Greece, for the period March to June 2012. A structured questionnaire containing demographic and clinical data was developed in order to collect retrospective data on the cases. Eye swab specimens were collected and molecular detection of adenoviruses was performed by nested PCR. Positive results were confirmed by sequencing. To determine the relatedness between the isolated sequences, a phylogenetic analysis was conducted. The epidemiological analysis (including retrospective data) included 231 conjunctivitis cases (47.1% male, and 52.8% female). Based on clinical features 205 of the cases were diagnosed of viral origin (46.3% male and 53.7% female), 4 of bacterial origin (50% male and 50% female) while 22 were undefined conjunctivitis. The outbreak excess cases (included 156 cases) affected all age groups regardless gender predilection. For the positive samples indicated that 29 samples (72.5%) were AdV17, and 5 (12.5%) as AdV54. Molecular analysis could define the cause of viral conjunctivitis, while epidemiological data contributed to the assessment of the risk factors and underlined possible preventive measures. This study is one of the very few on viral conjunctivitis in Greece. This outbreak underscores the need for a national surveillance system for acute infectious conjunctivitis outbreaks. The epidemiological as well as molecular investigation on HAdV ocular infections is rather absent in Greece, which has no surveillance system for viral conjunctivitis.Keywords
This publication has 28 references indexed in Scilit:
- Molecular evolution of human adenovirusesScientific Reports, 2013
- Ophthalmic Parasitosis: A Review ArticleInterdisciplinary Perspectives on Infectious Diseases, 2012
- Surveillance of Adenovirus D in patients with epidemic keratoconjunctivitis from Fukui Prefecture, Japan, 1995–2010Journal of Medical Virology, 2011
- Complete Genome Sequence of Human Adenovirus Prototype 17Journal of Virology, 2011
- Molecular detection of multiple viral targets in untreated urban sewage from GreeceVirology Journal, 2011
- Adenovirus Infections in Hospitalized Patients in Israel: Epidemiology and Molecular CharacterizationJournal of Clinical Microbiology, 2011
- Molecular epidemiology of adenoviral conjunctivitis in Athens, GreeceActa Ophthalmologica, 2010
- Spread of Epidemic Keratoconjunctivitis Due to a Novel Serotype of Human Adenovirus in JapanJournal of Clinical Microbiology, 2009
- Novel Human Adenovirus Causing Nosocomial Epidemic KeratoconjunctivitisJournal of Clinical Microbiology, 2008
- Molecular epidemiology of adenovirus strains isolated from patients with ocular disease in the area of Thessaloniki, Greece (1998-2002)Journal of Medical Virology, 2005