A single magnetic field exposure system for sequential investigation of real time and downstream cellular responses
- 1 January 2003
- journal article
- research article
- Published by Wiley in Bioelectromagnetics
- Vol. 25 (1), 27-32
- https://doi.org/10.1002/bem.10151
Abstract
To be able to correlate real time membrane potential or ion flux changes with further downstream gene transcription responses due to extremely low frequency (ELF) electromagnetic field (EMF) exposure, we devised an experimental system consisting of a pair of symmetric circular coils. This system can be used on an inverted microscope stage (real time signaling) as well as inside controlled environment incubators (gene transcription end points). The system includes a unique, custom made switch box for blinding the experimental staff and a power amplifier. We report herein the design and characterization of the system with respect to parameters considered important in in vitro ELF-EMF exposure studies, including linear magnetic field distribution, compensation for microscope objective lens interference, heating effects of the coils, and harmonic content of the signals.Keywords
This publication has 11 references indexed in Scilit:
- Effects of 60 Hz electromagnetic field exposure on APP695 transcription levels in differentiating human neuroblastoma cellsBioelectrochemistry, 2002
- Heat shock response: presence and effects in burn patient neutrophilsJournal of Leukocyte Biology, 1999
- Effects of microscope objectives on magnetic field exposuresBioelectromagnetics, 1999
- Kinetics of gene expression following exposure to 60 Hz, 2 mT magnetic fields in three human cell linesBioelectrochemistry and Bioenergetics, 1997
- Electromagnetic field stimulation of biosynthesis: changes in c-myc transcript levels during continuous and intermittent exposuresBioelectrochemistry and Bioenergetics, 1996
- Effects of Electromagnetic Fields on Molecules and CellsInternational Review of Cytology, 1995
- Design and characterization of a system for exposure of cultured cells to extremely low frequency electric and magnetic fields over a wide range of field strengthsBioelectromagnetics, 1993
- Biological, physical, and electrical parameters for in vitro studies with ELF magnetic and electric fields: A primerBioelectromagnetics, 1993
- ELF in vitro exposure systems for inducing uniform electric and magnetic fields in cell culture mediaBioelectromagnetics, 1992
- Exposure of human cells to low-frequency electromagnetic fields results in quantitative changes in transcriptsBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1989