Cutting Edge: A Thymocyte-Thymic Epithelial Cell Cross-Talk Dynamically Regulates Intrathymic IL-7 Expression In Vivo
- 1 June 2010
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 184 (11), 5949-5953
- https://doi.org/10.4049/jimmunol.1000601
Abstract
Thymic epithelial cells (TECs) are the predominant intrathymic source of the essential thymopoietin IL-7. Whether thymocyte-TEC interactions have a role in the regulation of IL-7 expression is not known. By exploiting IL-7 reporter mice in which yellow fluorescent protein expression identifies TECs expressing high levels of IL-7 (Il7+ TECs), we show that Il7+ TECs segregate from emerging medullary TECs during thymic organogenesis. Although Il7+ TECs normally diminish with age, we found that Il7+ TECs are markedly retained in alymphoid Rag2−/−Il2rg−/− IL-7 reporter mice that manifest a profound thymopoietic arrest. Transfer of Tcra−/− or wild-type (but not Rag2−/−) hematopoietic progenitors to alymphoid IL-7 reporter recipients normalizes the frequency of Il7+ TECs and re-establishes cortical TEC/medullary TEC segregation. Although thymocyte-derived signals are often considered stimulatory for TEC maturation, our findings identify a negative feedback mechanism in which signals derived from TCRβ-selected thymocytes modulate TEC-dependent IL-7 expression.This publication has 18 references indexed in Scilit:
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