Tandem Mass Spectrometry in Physiology
Open Access
- 1 December 2007
- journal article
- review article
- Published by American Physiological Society in Physiology
- Vol. 22 (6), 390-400
- https://doi.org/10.1152/physiol.00025.2007
Abstract
Tandem mass spectrometry coupled to liquid chromatography (LC-MS/MS) allows identification of proteins in a complex mixture without need for protein purification (“shotgun” proteomics). Recent progress in LC-MS/MS-based quantification, phosphoproteomic analysis, and targeted LC-MS/MS using multiple reaction monitoring (MRM) has made LC-MS/MS a powerful tool for the study of cell physiology.Keywords
This publication has 36 references indexed in Scilit:
- Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networksProceedings of the National Academy of Sciences, 2007
- Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometryNature Methods, 2007
- Direct interaction with filamins modulates the stability and plasma membrane expression of CFTRJCI Insight, 2007
- Large-scale phosphorylation analysis of mouse liverProceedings of the National Academy of Sciences of the United States of America, 2007
- LC-MS/MS Analysis of Apical and Basolateral Plasma Membranes of Rat Renal Collecting Duct CellsMolecular & Cellular Proteomics, 2006
- The utility of ETD mass spectrometry in proteomic analysisBiochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2006
- Quantitative phosphoproteomics of vasopressin-sensitive renal cells: Regulation of aquaporin-2 phosphorylation at two sitesProceedings of the National Academy of Sciences of the United States of America, 2006
- Proteome survey reveals modularity of the yeast cell machineryNature, 2006
- Multiplexed Protein Quantitation in Saccharomyces cerevisiae Using Amine-reactive Isobaric Tagging ReagentsMolecular & Cellular Proteomics, 2004
- The evolution of molecular biology into systems biologyNature Biotechnology, 2004